Cellosaurus H9 AAVS1-TRE3G-NGN2 Flag-EEA1 TMEM9-/- (CVCL_E2VX)
| Cell line name | H9 AAVS1-TRE3G-NGN2 Flag-EEA1 TMEM9-/- | |
|---|---|---|
| Synonyms | H9 ES AAVS1-NGN2 Flag-EEA1 TMEM9-/- | |
| Accession | CVCL_E2VX | |
| Resource Identification Initiative | To cite this cell line use: H9 AAVS1-TRE3G-NGN2 Flag-EEA1 TMEM9-/- (RRID:CVCL_E2VX) | |
| Comments | Characteristics: Using CRISPR/Cas9 a TRE3G promoter driven NEUROG2 construct was introduced in the AAVS1 safe harbor locus (from parent cell line). Characteristics: Using CRISPR/Cas9 EAA1 was N-terminally tagged on both alleles with a 3xFLAG tag (from parent cell line). Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:18823; TMEM9. Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:13805; NEUROG2. Miscellaneous: Cell line information from personal communication of Harper, Jeffrey Wade. Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. | |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) | |
| Hierarchy | Parent: CVCL_D1KV (H9 AAVS1-TRE3G-NGN2 3xFLAG-EEA1) Children:
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| Sex of cell | Female | |
| Age at sampling | Blastocyst stage | |
| Category | Embryonic stem cell | |
| Entry history | ||
| Entry creation | 10-Sep-2024 | |
| Last entry update | 19-Dec-2024 | |
| Version number | 2 | |