Cellosaurus logo
expasy logo

Cellosaurus H9 AAVS1-TRE3G-NGN2 3xFLAG-EEA1 (CVCL_D1KV)

[Text version]
Cell line name H9 AAVS1-TRE3G-NGN2 3xFLAG-EEA1
Synonyms H9 ES AAVS1-NGN2 3xFLAG-EEA1
Accession CVCL_D1KV
Resource Identification Initiative To cite this cell line use: H9 AAVS1-TRE3G-NGN2 3xFLAG-EEA1 (RRID:CVCL_D1KV)
Comments Characteristics: Using CRISPR/Cas9 a TRE3G promoter driven NEUROG2 construct was introduced in the AAVS1 safe harbor locus (from parent cell line).
Characteristics: Using CRISPR/Cas9 EAA1 was N-terminally tagged on both alleles with a 3xFLAG tag (Direct_author_submission).
Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:13805; NEUROG2.
Miscellaneous: Cell line information from personal communication of Harper, Jeffrey Wade.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_C4EK (H9 AAVS1-TRE3G-NGN2)
Children:
CVCL_E2VV (H9 AAVS1-TRE3G-NGN2 3xFLAG-EEA1 TMEM230-/-)CVCL_E2VW (H9 AAVS1-TRE3G-NGN2 Flag-EEA1 TMEM230 X121W)CVCL_E2VX (H9 AAVS1-TRE3G-NGN2 Flag-EEA1 TMEM9-/-)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Cross-references
Encyclopedic resources Wikidata; Q127380982
Entry history
Entry creation30-Jan-2024
Last entry update19-Dec-2024
Version number3