Cellosaurus H9 AAVS1-TRE3G-NGN2 Flag-EEA1 TMEM9-/-; TMEM9B-/- (CVCL_E2VY)
| Cell line name | H9 AAVS1-TRE3G-NGN2 Flag-EEA1 TMEM9-/-; TMEM9B-/- |
|---|---|
| Synonyms | H9 ES AAVS1-NGN2 Flag-EEA1 TMEM9-/-;TMEM9B-/- |
| Accession | CVCL_E2VY |
| Resource Identification Initiative | To cite this cell line use: H9 AAVS1-TRE3G-NGN2 Flag-EEA1 TMEM9-/-; TMEM9B-/- (RRID:CVCL_E2VY) |
| Comments | Characteristics: Using CRISPR/Cas9 a TRE3G promoter driven NEUROG2 construct was introduced in the AAVS1 safe harbor locus (from parent cell line). Characteristics: Using CRISPR/Cas9 EAA1 was N-terminally tagged on both alleles with a 3xFLAG tag (from parent cell line). Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:18823; TMEM9. Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:1168; TMEM9B. Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:13805; NEUROG2. Miscellaneous: Cell line information from personal communication of Harper, Jeffrey Wade. Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_E2VX (H9 AAVS1-TRE3G-NGN2 Flag-EEA1 TMEM9-/-) |
| Sex of cell | Female |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Entry history | |
| Entry creation | 10-Sep-2024 |
| Last entry update | 19-Dec-2024 |
| Version number | 2 |