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Cellosaurus CHSE-EC (CVCL_DG46)

[Text version]
Cell line name CHSE-EC
Synonyms EC
Accession CVCL_DG46
Resource Identification Initiative To cite this cell line use: CHSE-EC (RRID:CVCL_DG46)
Comments Group: Fish cell line.
Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=Nuclear version; nCas9n).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Cell type: Epithelial cell; CL=CL_0000066.
Species of origin Oncorhynchus tshawytscha (Chinook salmon) (Salmo tshawytscha) (NCBI Taxonomy: 74940)
Hierarchy Parent: CVCL_DG45 (CHSE-E)
Children:
CVCL_E3AE (CHSE-EC-PKR-C19)CVCL_E3AF (CHSE-EC-PKR-C28)CVCL_E3AC (CHSE-EC-PKR-C3)
CVCL_E3AD (CHSE-EC-PKR-C5)CVCL_YI85 (CHSE-GS2)
Sex of cell Sex unspecified
Age at sampling Embryo
Category Spontaneously immortalized cell line
Publications

PubMed=27236514; DOI=10.1007/s10126-016-9708-6; PMCID=PMC5007268
Dehler C.E., Boudinot P., Martin S.A.M., Collet B.
Development of an efficient genome editing method by CRISPR/Cas9 in a fish cell line.
Mar. Biotechnol. 18:449-452(2016)

PubMed=32576161; DOI=10.1186/s12896-020-00626-x; PMCID=PMC7310381
Gratacap R.L., Regan T., Dehler C.E., Martin S.A.M., Boudinot P., Collet B., Houston R.D.
Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery system.
BMC Biotechnol. 20:35.1-35.9(2020)

Cross-references
Encyclopedic resources Wikidata; Q54813318
Entry history
Entry creation13-Jul-2016
Last entry update10-Sep-2024
Version number11