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Cellosaurus CHSE-GS2 (CVCL_YI85)

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Cell line name CHSE-GS2
Synonyms GS2
Accession CVCL_YI85
Resource Identification Initiative To cite this cell line use: CHSE-GS2 (RRID:CVCL_YI85)
Comments Group: Fish cell line.
Characteristics: The parent cell line was transiently transfected with a mix of sgRNAs designed against stat2 and against mEGFP. mEGFP-deficient cells were isolated.
Knockout cell: Method=CRISPR/Cas9; UniProtKB; A0A8C8HVT5; stat2.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (nuclear version; nCas9n).
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Cell type: Epithelial cell; CL=CL_0000066.
Species of origin Oncorhynchus tshawytscha (Chinook salmon) (Salmo tshawytscha) (NCBI Taxonomy: 74940)
Hierarchy Parent: CVCL_DG46 (CHSE-EC)
Sex of cell Sex unspecified
Age at sampling Embryo
Category Spontaneously immortalized cell line
Publications

PubMed=31142600; DOI=10.4049/jimmunol.1801376
Dehler C.E., Lester K., Della Pelle G., Jouneau L., Houel A., Collins C., Dovgan T., Machat R., Zou J., Boudinot P., Martin S.A.M., Collet B.
Viral resistance and IFN signaling in STAT2 knockout fish cells.
J. Immunol. 203:465-475(2019)

Cross-references
Encyclopedic resources Wikidata; Q93453361
Entry history
Entry creation19-Dec-2019
Last entry update30-Jan-2024
Version number6