Cellosaurus CHSE-EC-PKR-C5 (CVCL_E3AD)
| Cell line name | CHSE-EC-PKR-C5 |
|---|---|
| Synonyms | EC-PKR-C5 |
| Accession | CVCL_E3AD |
| Resource Identification Initiative | To cite this cell line use: CHSE-EC-PKR-C5 (RRID:CVCL_E3AD) |
| Comments | Group: Fish cell line. Characteristics: The parent cell line was transiently transfected with a mix of sgRNAs designed against eif2ak2/pkr and against mEGFP. mEGFP-deficient cells were isolated (PubMed=39058317). Knockout cell: Method=CRISPR/Cas9; UniProtKB; A0A8C7HVT8; eif2ak2. Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transfection; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=Nuclear version; nCas9n). Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922. Cell type: Epithelial cell; CL=CL_0000066. |
| Species of origin | Oncorhynchus tshawytscha (Chinook salmon) (Salmo tshawytscha) (NCBI Taxonomy: 74940) |
| Hierarchy | Parent: CVCL_DG46 (CHSE-EC) |
| Sex of cell | Sex unspecified |
| Age at sampling | Embryo |
| Category | Spontaneously immortalized cell line |
| Publications | PubMed=39058317; DOI=10.4049/jimmunol.2400076 |
| Entry history | |
| Entry creation | 10-Sep-2024 |
| Last entry update | 10-Sep-2024 |
| Version number | 1 |