ID   UMANe002-A-2
AC   CVCL_D6JR
DR   BioSamples; SAMEA114295974
DR   hPSCreg; UMANe002-A-2
DR   Wikidata; Q127384582
RX   PubMed=38100908;
CC   From: University of Manchester; Manchester; United Kingdom.
CC   Population: Caucasian.
CC   Characteristics: Produces a robust fluorescent signal in cardiac cells and have wide utility particularly for research on developmental biology and disease modelling (PubMed=38100908).
CC   Characteristics: Using CRISPR/Cas9 a T2A-eGFP construct was integrated at the 3'end of one allele of NKX2-5 (PubMed=38100908).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_L201 ! Man-13
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 02-05-24; Last updated: 19-12-24; Version: 3
CH   CVCL_E3RR ! UMANe002-A-3
//
RX   PubMed=38100908; DOI=10.1016/j.scr.2023.103262;
RA   Douglas, Megan
RA   O'Loughlin, Chloe
RA   Lynch, Adam T.
RA   Prialgauskaite, Radvile
RA   Adamson, Anthony D.
RA   Dibb, Katharine M.
RA   Kimber, Susan J.
RA   Birket, Matthew J.
RT   "The generation and validation of two NKX2-5 fluorescent reporter
RT   human embryonic stem cell lines: UMANe002-A-1 and UMANe002-A-2.";
RL   Stem Cell Res. 74:103262-103262(2024).
//