ID   UMANe002-A-2
AC   CVCL_D6JR
DR   BioSamples; SAMEA114295974
DR   hPSCreg; UMANe002-A-2
DR   Wikidata; Q127384582
RX   PubMed=38100908;
CC   Characteristics: Produces a robust fluorescent signal in cardiac cells and have wide utility particularly for research on developmental biology and disease modelling (PubMed=38100908).
CC   Characteristics: Using CRISPR/Cas9 a T2A-eGFP construct was integrated at the 3'end of one allele of NKX2-5 (PubMed=38100908).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_L201 ! Man-13
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 02-05-24; Last updated: 10-09-24; Version: 2
//
RX   PubMed=38100908; DOI=10.1016/j.scr.2023.103262;
RA   Douglas M., O'Loughlin C., Lynch A.T., Prialgauskaite R.,
RA   Adamson A.D., Dibb K.M., Kimber S.J., Birket M.J.;
RT   "The generation and validation of two NKX2-5 fluorescent reporter
RT   human embryonic stem cell lines: UMANe002-A-1 and UMANe002-A-2.";
RL   Stem Cell Res. 74:103262-103262(2024).
//