Cellosaurus MDCK-EKARrEV-NLS-dEGFR (CVCL_D5IW)
| Cell line name | MDCK-EKARrEV-NLS-dEGFR |
|---|---|
| Accession | CVCL_D5IW |
| Resource Identification Initiative | To cite this cell line use: MDCK-EKARrEV-NLS-dEGFR (RRID:CVCL_D5IW) |
| Comments | Characteristics: Transduced with the FRET biosensor EKARrEV-NLS FRET biosensor EKARrEV-NLS, which consists of a donor fluorescent protein CFP, an ERK substrate peptide derived from RSK1, an optimized linker, a FHA1 phosphate binding domain, an acceptor fluorescent protein YFP and a nuclear localization signal (PubMed=34667080). Knockout cell: Method=CRISPR/Cas9; VGNC; 51866; EGFR. Genetic integration: Method=Transduction; Gene=FPbase; ZRLU4; CFP (Note=Cyan fluorescent protein derived from GFP). Genetic integration: Method=Transduction; Gene=FPbase; 8DNLG; YFP (Note=EYFP, yellow fluorescent protein derived from GFP). Derived from site: In situ; Kidney; UBERON=UBERON_0002113. Cell type: Epithelial cell of kidney; CL=CL_0002518. |
| Species of origin | Canis lupus familiaris (Dog) (Canis familiaris)
(NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372. |
| Hierarchy | Parent: CVCL_D5IR (MDCK-EKARrEV-NLS) |
| Sex of cell | Female |
| Age at sampling | Adult |
| Category | Spontaneously immortalized cell line |
| Publications | PubMed=34667080; DOI=10.26508/lsa.202101206; PMCID=PMC8548211 |
| Cross-references | |
| Cell line collections (Providers) | JCRB; JCRB1994 |
| Encyclopedic resources | Wikidata; Q127382786 |
| Entry history | |
| Entry creation | 02-May-2024 |
| Last entry update | 10-Sep-2024 |
| Version number | 2 |