Cellosaurus BY-2 (CVCL_D5GM)
Cell line name | BY-2 | |||||
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Synonyms | BY2; TBY-2; TBY2; Tobacco BY-2; NT-1; NT1; Nicotiana tabacum-1 | |||||
Accession | CVCL_D5GM | |||||
Resource Identification Initiative | To cite this cell line use: BY-2 (RRID:CVCL_D5GM) | |||||
Comments | Group: Plant cell line. Characteristics: Can be used to prepare protoplasts (PubMed=17947977). Characteristics: Produces low levels of nicotine (PubMed=18567891). Characteristics: Tested for the production of animal proteins (PubMed=7766897). Characteristics: Tested for the production of bacterial proteins (PubMed=14688094; PubMed=22928883). Characteristics: Tested for the production of synthetic proteins (PubMed=15818563). Characteristics: Tested for the production of viral proteins (PubMed=14680934; PubMed=12601177; PubMed=16187337). Characteristics: Used as model for the electrochemical sensing of beta-glucuronidase activity of transgenic cell lines (PubMed=29982119). Doubling time: 15.8 hours (DOI=10.1016/S0074-7696(08)62452-3). Omics: Transcriptome analysis by microarray. Omics: Transcriptome analysis by RNAseq. Anecdotal: BY-2 cell line was generated at the Japan Tobacco and Salt Monopoly Public Corporation. In 1982 Toshiyuki Nagata brought it with him to the USA and left it there after renaming it to NT-1 in order to distribute it clandestinely because he was not allowed officially to give the BY-2 cells to others. He called it NT-1 as it could both mean Nicotiana tabacum or Nagata, Toshiyuki (PubMed=37642778). Derived from site: In situ; Seedling; PO=PO_0008037. | |||||
Species of origin | Nicotiana tabacum (Common tobacco)
(NCBI Taxonomy: 4097)
Breed/subspecies: Cultivar Bright Yellow 2. | |||||
Hierarchy | Children:
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Category | Spontaneously immortalized cell line | |||||
Web pages | https://en.wikipedia.org/wiki/Tobacco_BY-2_cells | |||||
Publications | CLPUB00765 PubMed=16664453; DOI=10.1104/pp.79.2.568; PMCID=PMC1074928 DOI=10.1016/S0074-7696(08)62452-3 PubMed=7766897; DOI=10.1007/BF00020889 PubMed=10728644; DOI=10.1023/A:1009832822096 PubMed=12601177; DOI=10.1073/pnas.0438037100; PMCID=PMC151319 PubMed=14680934; DOI=10.1016/j.pep.2003.07.004 PubMed=14688094; DOI=10.1128/IAI.72.1.168-175.2004; PMCID=PMC343997 PubMed=15638240; DOI=10.1007/s11745-004-1289-0 PubMed=15818563; DOI=10.1002/bit.20441 DOI=10.1007/3-540-32674-X_8 PubMed=16187337; DOI=10.1002/bit.20695 PubMed=17406390; DOI=10.1038/nprot.2006.176 PubMed=17406517; DOI=10.1038/nprot.2006.381 PubMed=17947977; DOI=10.1038/nprot.2007.360 PubMed=18567891; DOI=10.1093/pcp/pcn096 DOI=10.4067/S0718-07642010000200002 PubMed=22055919; DOI=10.1016/j.jbiosc.2011.09.022 PubMed=22928883; DOI=10.1080/03079457.2012.709606 PubMed=29982119; DOI=10.1016/j.bios.2018.06.045 PubMed=37642778; DOI=10.1007/s10265-023-01490-4; PMCID=PMC10587202 | |||||
Cross-references | ||||||
Cell line collections (Providers) | DSMZ; PC-1181 - Discontinued
RIKEN_BRC_EPD; rpc00001 | |||||
Anatomy/cell type resources | BTO; BTO_0001972 | |||||
Encyclopedic resources | Wikidata; Q7811455 | |||||
Experimental variables resources | EFO; EFO_0022754 | |||||
Gene expression databases | GEO; GSE6948
GEO; GSE6949 GEO; GSE14250 GEO; GSE37649 GEO; GSE42562 GEO; GSE111980 GEO; GSE160438 GEO; GSE193131 | |||||
Entry history | ||||||
Entry creation | 02-May-2024 | |||||
Last entry update | 19-Dec-2024 | |||||
Version number | 3 |