ID   BY-2
AC   CVCL_D5GM
SY   BY2; TBY-2; TBY2; Tobacco BY-2; NT-1; NT1; Nicotiana tabacum-1
DR   BTO; BTO:0001972
DR   EFO; EFO_0022754
DR   DSMZ; PC-1181
DR   GEO; GSE6948
DR   GEO; GSE6949
DR   GEO; GSE14250
DR   GEO; GSE37649
DR   GEO; GSE42562
DR   GEO; GSE111980
DR   GEO; GSE160438
DR   GEO; GSE193131
DR   RIKEN_BRC_EPD; rpc00001
DR   Wikidata; Q7811455
RX   CelloPub=CLPUB00765;
RX   DOI=10.1007/3-540-32674-X_8;
RX   DOI=10.1016/S0074-7696(08)62452-3;
RX   DOI=10.4067/S0718-07642010000200002;
RX   PubMed=7766897;
RX   PubMed=10728644;
RX   PubMed=12601177;
RX   PubMed=14680934;
RX   PubMed=14688094;
RX   PubMed=15638240;
RX   PubMed=15818563;
RX   PubMed=16187337;
RX   PubMed=16664453;
RX   PubMed=17406390;
RX   PubMed=17406517;
RX   PubMed=17947977;
RX   PubMed=18567891;
RX   PubMed=22055919;
RX   PubMed=22928883;
RX   PubMed=29982119;
RX   PubMed=37642778;
WW   https://en.wikipedia.org/wiki/Tobacco_BY-2_cells
CC   Group: Plant cell line.
CC   Characteristics: Can be used to prepare protoplasts (PubMed=17947977).
CC   Characteristics: Produces low levels of nicotine (PubMed=18567891).
CC   Characteristics: Tested for the production of animal proteins (PubMed=7766897).
CC   Characteristics: Tested for the production of bacterial proteins (PubMed=14688094; PubMed=22928883).
CC   Characteristics: Tested for the production of synthetic proteins (PubMed=15818563).
CC   Characteristics: Tested for the production of viral proteins (PubMed=14680934; PubMed=12601177; PubMed=16187337).
CC   Characteristics: Used as model for the electrochemical sensing of beta-glucuronidase activity of transgenic cell lines (PubMed=29982119).
CC   Doubling time: 15.8 hours (DOI=10.1016/S0074-7696(08)62452-3).
CC   Omics: Transcriptome analysis by microarray.
CC   Omics: Transcriptome analysis by RNAseq.
CC   Anecdotal: BY-2 cell line was generated at the Japan Tobacco and Salt Monopoly Public Corporation. In 1982 Toshiyuki Nagata brought it with him to the USA and left it there after renaming it to NT-1 in order to distribute it clandestinely because he was not allowed officially to give the BY-2 cells to others. He called it NT-1 as it could both mean Nicotiana tabacum or Nagata, Toshiyuki (PubMed=37642778).
CC   Discontinued: DSMZ; PC-1181; true.
CC   Derived from site: In situ; Seedling; PO=PO_0008037.
CC   Breed/subspecies: Cultivar Bright Yellow 2.
OX   NCBI_TaxID=4097; ! Nicotiana tabacum (Common tobacco)
CA   Spontaneously immortalized cell line
DT   Created: 02-05-24; Last updated: 10-09-24; Version: 2
//
RX   CelloPub=CLPUB00765;
RA   Kato K., Matsumoto T., Koiwai A., Mizusaki S., Nishida K.,
RA   Noguchi M., Tamaki E.;
RT   "Liquid suspension culture of tobacco cells.";
RL   (In book chapter) Fermentation technology today. Proceedings of the IVth international fermentation symposium; Terui G. (eds.); pp.689-695; Society of Fermentation Technology; Osaka; Japan (1972).
//
RX   DOI=10.1007/3-540-32674-X_8;
RA   Petrasek J., Zazimalova E.;
RT   "The BY-2 cell line as a tool to study auxin transport.";
RL   Biotechnol. Agric. For. 58:107-117(2006).
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RX   DOI=10.1016/S0074-7696(08)62452-3;
RA   Nagata T., Nemoto Y., Hasezawa S.;
RT   "Tobacco BY-2 cell line as the HeLa cell in the cell biology of higher
RT   plants.";
RL   Int. Rev. Cytol. 132:1-30(1992).
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RX   DOI=10.4067/S0718-07642010000200002;
RA   Ruiz L.F., Higareda A.E., Pardo M.A.;
RT   "Synchronization of tobacco cells (Nicotiana tabacum) NT-1.";
RL   Inf. Tecnol. 21:3-11(2010).
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RX   PubMed=7766897; DOI=10.1007/BF00020889;
RA   Matsumoto S., Ikura K., Ueda M., Sasaki R.;
RT   "Characterization of a human glycoprotein (erythropoietin) produced in
RT   cultured tobacco cells.";
RL   Plant Mol. Biol. 27:1163-1172(1995).
//
RX   PubMed=10728644; DOI=10.1023/A:1009832822096;
RA   Nagata T., Kumagai F.;
RT   "Plant cell biology through the window of the highly synchronized
RT   tobacco BY-2 cell line.";
RL   Methods Cell Sci. 21:123-127(1999).
//
RX   PubMed=12601177; DOI=10.1073/pnas.0438037100; PMCID=PMC151319;
RA   Sojikul P., Buehner N., Mason H.S.;
RT   "A plant signal peptide-hepatitis B surface antigen fusion protein
RT   with enhanced stability and immunogenicity expressed in plant
RT   cells.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:2209-2214(2003).
//
RX   PubMed=14680934; DOI=10.1016/j.pep.2003.07.004;
RA   Sunil Kumar G.B., Ganapathi T.R., Revathi C.J., Prasad K.S.,
RA   Bapat V.A.;
RT   "Expression of hepatitis B surface antigen in tobacco cell suspension
RT   cultures.";
RL   Protein Expr. Purif. 32:10-17(2003).
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RX   PubMed=14688094; DOI=10.1128/IAI.72.1.168-175.2004; PMCID=PMC343997;
RA   Judge N.A., Mason H.S., O'Brien A.D.;
RT   "Plant cell-based intimin vaccine given orally to mice primed with
RT   intimin reduces time of Escherichia coli O157:H7 shedding in feces.";
RL   Infect. Immun. 72:168-175(2004).
//
RX   PubMed=15638240; DOI=10.1007/s11745-004-1289-0;
RA   Hemmerlin A., Gerber E., Feldtrauer J.-F., Wentzinger L.,
RA   Hartmann M.-A., Tritsch D., Hoeffler J.-F., Rohmer M., Bach T.J.;
RT   "A review of tobacco BY-2 cells as an excellent system to study the
RT   synthesis and function of sterols and other isoprenoids.";
RL   Lipids 39:723-735(2004).
//
RX   PubMed=15818563; DOI=10.1002/bit.20441;
RA   Xu J.-F., Shpak E., Gu T.-Y., Moo-Young M., Kieliszewski M.J.;
RT   "Production of recombinant plant gum with tobacco cell culture in
RT   bioreactor and gum characterization.";
RL   Biotechnol. Bioeng. 90:578-588(2005).
//
RX   PubMed=16187337; DOI=10.1002/bit.20695;
RA   Zhang X.-R., Mason H.S.;
RT   "Bean yellow dwarf virus replicons for high-level transgene expression
RT   in transgenic plants and cell cultures.";
RL   Biotechnol. Bioeng. 93:271-279(2006).
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RX   PubMed=16664453; DOI=10.1104/pp.79.2.568; PMCID=PMC1074928;
RA   An G.;
RT   "High efficiency transformation of cultured tobacco cells.";
RL   Plant Physiol. 79:568-570(1985).
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RX   PubMed=17406390; DOI=10.1038/nprot.2006.176;
RA   Mayo K.J., Gonzales B.J., Mason H.S.;
RT   "Genetic transformation of tobacco NT1 cells with Agrobacterium
RT   tumefaciens.";
RL   Nat. Protoc. 1:1105-1111(2006).
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RX   PubMed=17406517; DOI=10.1038/nprot.2006.381;
RA   Kumagai-Sano F., Hayashi T., Sano T., Hasezawa S.;
RT   "Cell cycle synchronization of tobacco BY-2 cells.";
RL   Nat. Protoc. 1:2621-2627(2006).
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RX   PubMed=17947977; DOI=10.1038/nprot.2007.360;
RA   Miao Y.-S., Jiang L.-W.;
RT   "Transient expression of fluorescent fusion proteins in protoplasts of
RT   suspension cultured cells.";
RL   Nat. Protoc. 2:2348-2353(2007).
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RX   PubMed=18567891; DOI=10.1093/pcp/pcn096;
RA   Shoji T., Hashimoto T.;
RT   "Why does anatabine, but not nicotine, accumulate in
RT   jasmonate-elicited cultured tobacco BY-2 cells?";
RL   Plant Cell Physiol. 49:1209-1216(2008).
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RX   PubMed=22055919; DOI=10.1016/j.jbiosc.2011.09.022;
RA   Ullisch D.A., Muller C.A., Maibaum S., Kirchhoff J., Schiermeyer A.,
RA   Schillberg S., Roberts J.L., Treffenfeldt W., Buchs J.;
RT   "Comprehensive characterization of two different Nicotiana tabacum
RT   cell lines leads to doubled GFP and HA protein production by media
RT   optimization.";
RL   J. Biosci. Bioeng. 113:242-248(2012).
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RX   PubMed=22928883; DOI=10.1080/03079457.2012.709606;
RA   Miller T., Fanton M., Nickelson S., Mason H.S., Webb S.;
RT   "Safety and immunogenicity of bacterial and tobacco plant cell line
RT   derived recombinant native and mutant Escherichia coli heat-labile
RT   toxin in chickens.";
RL   Avian Pathol. 41:441-449(2012).
//
RX   PubMed=29982119; DOI=10.1016/j.bios.2018.06.045;
RA   Pandey R., Teig-Sussholz O., Schuster S., Avni A., Shacham-Diamand Y.;
RT   "Integrated electrochemical Chip-on-Plant functional sensor for
RT   monitoring gene expression under stress.";
RL   Biosens. Bioelectron. 117:493-500(2018).
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RX   PubMed=37642778; DOI=10.1007/s10265-023-01490-4; PMCID=PMC10587202;
RA   Nagata T.;
RT   "Hidden history of the tobacco BY-2 cell line.";
RL   J. Plant Res. 136:781-786(2023).
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