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Cellosaurus CH-RE1.22c-A3P2 (CVCL_D3WH)

[Text version]
Cell line name CH-RE1.22c-A3P2
Synonyms CH-RE1.22cA3P2
Accession CVCL_D3WH
Resource Identification Initiative To cite this cell line use: CH-RE1.22c-A3P2 (RRID:CVCL_D3WH)
Comments Characteristics: This is a class II diphtheria toxin resistant cell line. The resistance of class II mutants cannot be overcome by increased concentration of the toxin. They possess a mutationally altered EF-2 which does not serve as a substrate for ADP-ribosylation by diphtheria toxin (from parent cell line).
Selected for resistance to: UniProtKB; P00588; Corynephage beta diphtheria toxin.
Transformant: ChEBI; CHEBI:23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Mutation; UniProtKB; P09445; EEF2; Simple; p.Gly717Arg (c.2149G>A); Zygosity=Homozygous (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_D3WG (CH-RE1.22c)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=6928655; DOI=10.1073/pnas.77.2.1010; PMCID=PMC348413
Moehring J.M., Moehring T.J., Danley D.E.
Posttranslational modification of elongation factor 2 in diphtheria-toxin-resistant mutants of CHO-K1 cells.
Proc. Natl. Acad. Sci. U.S.A. 77:1010-1014(1980)

Cross-references
Encyclopedic resources Wikidata; Q127380447
Entry history
Entry creation30-Jan-2024
Last entry update10-Sep-2024
Version number2