Cellosaurus H9 AAVS1-TRE3G-NGN2 TMEM192-3xHA YIPF4-/- (CVCL_D1KU)
| Cell line name | H9 AAVS1-TRE3G-NGN2 TMEM192-3xHA YIPF4-/- |
|---|---|
| Synonyms | H9 ES AAVS1-NGN2 TMEM192-3xHA YIPF4-/- |
| Accession | CVCL_D1KU |
| Resource Identification Initiative | To cite this cell line use: H9 AAVS1-TRE3G-NGN2 TMEM192-3xHA YIPF4-/- (RRID:CVCL_D1KU) |
| Comments | Characteristics: Using CRISPR/Cas9 a TRE3G promoter driven NEUROG2 construct was introduced in the AAVS1 safe harbor locus (from parent cell line). Characteristics: Using CRISPR/Cas9 TMEM192 was C-terminally tagged on both alleles with a 3xHA epitope (PubMed=37757899). Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:28145; YIPF4. Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:13805; NEUROG2. Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_D1KT (H9 AAVS1-TRE3G-NGN2 TMEM192-3xHA) |
| Sex of cell | Female |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=37757899; DOI=10.1038/s41586-023-06657-6; PMCID=PMC10620096 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q127381014 |
| Entry history | |
| Entry creation | 30-Jan-2024 |
| Last entry update | 19-Dec-2024 |
| Version number | 3 |