ID   H9 AAVS1-TRE3G-NGN2 TMEM192-3xHA YIPF4-/-
AC   CVCL_D1KU
SY   H9 ES AAVS1-NGN2 TMEM192-3xHA YIPF4-/-
DR   Wikidata; Q127381014
RX   PubMed=37757899;
CC   Characteristics: Using CRISPR/Cas9 a TRE3G promoter driven NEUROG2 construct was introduced in the AAVS1 safe harbor locus (from parent cell line).
CC   Characteristics: Using CRISPR/Cas9 TMEM192 was C-terminally tagged on both alleles with a 3xHA epitope (PubMed=37757899).
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:28145; YIPF4.
CC   Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:13805; NEUROG2.
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_D1KT ! H9 AAVS1-TRE3G-NGN2 TMEM192-3xHA
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 30-01-24; Last updated: 19-12-24; Version: 3
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RX   PubMed=37757899; DOI=10.1038/s41586-023-06657-6; PMCID=PMC10620096;
RA   Hickey K.L., Swarup S., Smith I.R., Paoli J.C., Whelan E.M.,
RA   Paulo J.A., Harper J.W.;
RT   "Proteome census upon nutrient stress reveals Golgiphagy membrane
RT   receptors.";
RL   Nature 623:167-174(2023).
//