Cellosaurus H9 AAVS1-TRE3G-NGN2 TMEM192-3xHA (CVCL_D1KT)
| Cell line name | H9 AAVS1-TRE3G-NGN2 TMEM192-3xHA | ||||
|---|---|---|---|---|---|
| Synonyms | H9 ES AAVS1-NGN2 TMEM192-3xHA | ||||
| Accession | CVCL_D1KT | ||||
| Resource Identification Initiative | To cite this cell line use: H9 AAVS1-TRE3G-NGN2 TMEM192-3xHA (RRID:CVCL_D1KT) | ||||
| Comments | Characteristics: Using CRISPR/Cas9 a TRE3G promoter driven NEUROG2 construct was introduced in the AAVS1 safe harbor locus (from parent cell line). Characteristics: Using CRISPR/Cas9 TMEM192 was C-terminally tagged on both alleles with a 3xHA epitope (PubMed=37757899). Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:13805; NEUROG2. Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. | ||||
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) | ||||
| Hierarchy | Parent: CVCL_C4EK (H9 AAVS1-TRE3G-NGN2) Children:
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| Sex of cell | Female | ||||
| Age at sampling | Blastocyst stage | ||||
| Category | Embryonic stem cell | ||||
| Publications | PubMed=37757899; DOI=10.1038/s41586-023-06657-6; PMCID=PMC10620096 | ||||
| Cross-references | |||||
| Encyclopedic resources | Wikidata; Q127381012 | ||||
| Entry history | |||||
| Entry creation | 30-Jan-2024 | ||||
| Last entry update | 19-Dec-2024 | ||||
| Version number | 3 | ||||