Cellosaurus H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/- Keima-REEP5 (CVCL_D1IS)
| Cell line name | H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/- Keima-REEP5 |
|---|---|
| Synonyms | H9 ES AAVS1-NGN2 FAM134C/A-/-; PiggyBac-Keima-REEP5 |
| Accession | CVCL_D1IS |
| Resource Identification Initiative | To cite this cell line use: H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/- Keima-REEP5 (RRID:CVCL_D1IS) |
| Comments | Characteristics: Using CRISPR/Cas9 a TRE3G promoter driven NEUROG2 construct was introduced in the AAVS1 safe harbor locus (from parent cell line). Characteristics: Transposed with a PiggyBac transposon vector pAC150-PBLHL-4xHS-EF1a-Keima-REEP5 that localizes specifically to endoplasmic reticulum tubules (PubMed=38429475). Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:28450; RETREG2. Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:27258; RETREG3. Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:13805; NEUROG2. Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:30077; REEP5. Genetic integration: Method=PiggyBac transposition; Gene=FPbase; SXBT1; mKeima (Note=Long stokes shift fluorescent protein from Montipora sp. 20). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_D1IB (H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/-) |
| Sex of cell | Female |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=38429475; DOI=10.1038/s41556-024-01356-4; PMCID=PMC10940164 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q127380999 |
| Entry history | |
| Entry creation | 30-Jan-2024 |
| Last entry update | 19-Dec-2024 |
| Version number | 3 |