ID   H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/- Keima-REEP5
AC   CVCL_D1IS
SY   H9 ES AAVS1-NGN2 FAM134C/A-/-; PiggyBac-Keima-REEP5
DR   Wikidata; Q127380999
RX   PubMed=38429475;
CC   Characteristics: Using CRISPR/Cas9 a TRE3G promoter driven NEUROG2 construct was introduced in the AAVS1 safe harbor locus (from parent cell line).
CC   Characteristics: Transposed with a PiggyBac transposon vector pAC150-PBLHL-4xHS-EF1a-Keima-REEP5 that localizes specifically to endoplasmic reticulum tubules (PubMed=38429475).
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:28450; RETREG2.
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:27258; RETREG3.
CC   Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:13805; NEUROG2.
CC   Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:30077; REEP5.
CC   Genetic integration: Method=PiggyBac transposition; Gene=FPbase; SXBT1; mKeima (Note=Long stokes shift fluorescent protein from Montipora sp. 20).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_D1IB ! H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/-
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 30-01-24; Last updated: 19-12-24; Version: 3
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RX   PubMed=38429475; DOI=10.1038/s41556-024-01356-4; PMCID=PMC10940164;
RA   Hoyer M.J., Capitanio C., Smith I.R., Paoli J.C., Bieber A.,
RA   Jiang Y.-Z., Paulo J.A., Gonzalez-Lozano M.A., Baumeister W.P.,
RA   Wilfling F., Schulman B.A., Harper J.W.;
RT   "Combinatorial selective ER-phagy remodels the ER during
RT   neurogenesis.";
RL   Nat. Cell Biol. 26:378-392(2024).
//