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Cellosaurus iSLK (CVCL_B6YU)

[Text version]
Cell line name iSLK
Accession CVCL_B6YU
Resource Identification Initiative To cite this cell line use: iSLK (RRID:CVCL_B6YU)
Comments Problematic cell line: Contaminated. Shown to be a Caki-1 derivative (PubMed=22987579). The parent cell line was originally thought to originate from a 28 year old male patient with HIV-1 negative Kaposi sarcoma.
Population: Caucasian.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Derived from site: Metastatic; Skin; UBERON=UBERON_0002097.
Sequence variations
  • Mutation; HGNC; 9884; RB1; Simple; p.Leu98Pro (c.293T>C); ClinVar=VCV001502192; Zygosity=Heterozygous (from parent cell line).
Disease Clear cell renal cell carcinoma (NCIt: C4033)
Clear cell renal carcinoma (ORDO: Orphanet_319276)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9569 (SLK)
Children:
CVCL_B6YV (iSLK.219)
Sex of cell Male
Age at sampling 49Y
Category Cancer cell line
STR profile Source(s): PubMed=22987579

Markers:
AmelogeninX
CSF1PO11
D5S81811
D7S8208,12
D13S31711,12
D16S53912
TH016,8
TPOX8,11
vWA15,17

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Publications

PubMed=21419799; DOI=10.1016/j.jviromet.2011.03.012; PMCID=PMC3095772
Myoung J., Ganem D.E.
Generation of a doxycycline-inducible KSHV producer cell line of endothelial origin: maintenance of tight latency with efficient reactivation upon induction.
J. Virol. Methods 174:12-21(2011)

PubMed=22987579; DOI=10.1002/ijc.27849
Sturzl M., Gaus D., Dirks W.G., Ganem D.E., Jochmann R.
Kaposi's sarcoma-derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line.
Int. J. Cancer 132:1954-1958(2013)

Cross-references
Encyclopedic resources Wikidata; Q111733518
Entry history
Entry creation17-Mar-2022
Last entry update10-Sep-2024
Version number7