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Cellosaurus iSLK.219 (CVCL_B6YV)

[Text version]
Cell line name iSLK.219
Synonyms iSLK.219 clone #10
Accession CVCL_B6YV
Resource Identification Initiative To cite this cell line use: iSLK.219 (RRID:CVCL_B6YV)
Comments Problematic cell line: Contaminated. Shown to be a Caki-1 derivative (PubMed=22987579). The grand-parent cell line was originally thought to originate from a 28 year old male patient with HIV-1 negative Kaposi sarcoma.
Population: Caucasian.
Characteristics: rKSHV.219 includes a RFP-GFP-Puro construct that contains RFP under the control of the lytic PAN RNA promoter, GFP under the control of human EF-1 alpha promoter and PuroR expressed by the RSV promoter.
Characteristics: Transduced with pRetroX-Tet-On that contains a reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16 and with RetroX-Tight-Hyg-RTA.
Virology: Maintains latent rKSHV.219 as indicated by expression of constitutively expressed (eGFP) but not a lytic phase (RFP) reporter gene. When exposed to either sodium butyrate or doxycycline, the cells are activated to lytic replication and express RFP and KSHV lytic genes and release large quantities of infectious virus (PubMed=21419799).
Virology: Infected with a recombinant human herpesvirus 8 (HHV-8/KSHV) rKSHV.219.
Transfected with: UniProtKB; Q9U6Y8; RFP.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
Derived from site: Metastatic; Skin; UBERON=UBERON_0002097.
Sequence variations
  • Mutation; HGNC; 9884; RB1; Simple; p.Leu98Pro (c.293T>C); ClinVar=VCV001502192; Zygosity=Heterozygous (from parent cell line).
Disease Clear cell renal cell carcinoma (NCIt: C4033)
Clear cell renal carcinoma (ORDO: Orphanet_319276)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_B6YU (iSLK)
Sex of cell Male
Age at sampling 49Y
Category Cancer cell line
STR profile Source(s): PubMed=22987579

Markers:
AmelogeninX
CSF1PO11
D5S81811
D7S8208,12
D13S31711,12
D16S53912
TH016,8
TPOX8,11
vWA15,17

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Publications

PubMed=21419799; DOI=10.1016/j.jviromet.2011.03.012
Myoung J., Ganem D.
Generation of a doxycycline-inducible KSHV producer cell line of endothelial origin: maintenance of tight latency with efficient reactivation upon induction.
J. Virol. Methods 174:12-21(2011)

PubMed=22987579; DOI=10.1002/ijc.27849
Sturzl M., Gaus D., Dirks W.G., Ganem D., Jochmann R.
Kaposi's sarcoma-derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line.
Int. J. Cancer 132:1954-1958(2013)

Cross-references
Encyclopedic resources Wikidata; Q111733519
Entry history
Entry creation17-Mar-2022
Last entry update29-Jun-2023
Version number5