Cellosaurus iSLK.219 (CVCL_B6YV)
Cell line name | iSLK.219 | ||||||||||||||||||
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Synonyms | iSLK.219 clone #10 | ||||||||||||||||||
Accession | CVCL_B6YV | ||||||||||||||||||
Resource Identification Initiative | To cite this cell line use: iSLK.219 (RRID:CVCL_B6YV) | ||||||||||||||||||
Comments | Problematic cell line: Contaminated. Shown to be a Caki-1 derivative (PubMed=22987579). The grand-parent cell line was originally thought to originate from a 28 year old male patient with HIV-1 negative Kaposi sarcoma. Population: Caucasian. Characteristics: rKSHV.219 includes a RFP-GFP-Puro construct that contains RFP under the control of the lytic PAN RNA promoter, GFP under the control of human EF-1 alpha promoter and PuroR expressed by the RSV promoter. Characteristics: Transduced with pRetroX-Tet-On that contains a reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16 and with RetroX-Tight-Hyg-RTA. Virology: Maintains latent rKSHV.219 as indicated by expression of constitutively expressed (eGFP) but not a lytic phase (RFP) reporter gene. When exposed to either sodium butyrate or doxycycline, the cells are activated to lytic replication and express RFP and KSHV lytic genes and release large quantities of infectious virus (PubMed=21419799). Virology: Infected with a recombinant human herpesvirus 8 (HHV-8/KSHV) rKSHV.219. Transfected with: UniProtKB; Q9U6Y8; RFP. Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph). Transfected with: UniProtKB; P00552; Transposon Tn5 neo. Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). Derived from site: Metastatic; Skin; UBERON=UBERON_0002097. | ||||||||||||||||||
Sequence variations |
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Disease | Clear cell renal cell carcinoma (NCIt: C4033) Clear cell renal carcinoma (ORDO: Orphanet_319276) | ||||||||||||||||||
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) | ||||||||||||||||||
Hierarchy | Parent: CVCL_B6YU (iSLK) | ||||||||||||||||||
Sex of cell | Male | ||||||||||||||||||
Age at sampling | 49Y | ||||||||||||||||||
Category | Cancer cell line | ||||||||||||||||||
STR profile | Source(s): PubMed=22987579 Markers:
Run an STR similarity search on this cell line | ||||||||||||||||||
Publications | PubMed=21419799; DOI=10.1016/j.jviromet.2011.03.012 PubMed=22987579; DOI=10.1002/ijc.27849 | ||||||||||||||||||
Cross-references | |||||||||||||||||||
Encyclopedic resources | Wikidata; Q111733519 | ||||||||||||||||||
Entry history | |||||||||||||||||||
Entry creation | 17-Mar-2022 | ||||||||||||||||||
Last entry update | 29-Jun-2023 | ||||||||||||||||||
Version number | 5 |