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Cellosaurus BALB/3T3 clone A31 ts20 (CVCL_B385)

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Cell line name BALB/3T3 clone A31 ts20
Synonyms ts20; Ts20; TS-20; A31N-ts20; A31N ts20; A31Nts20
Accession CVCL_B385
Secondary accession CVCL_D594
Resource Identification Initiative To cite this cell line use: BALB/3T3 clone A31 ts20 (RRID:CVCL_B385)
Comments Characteristics: Temperature sensitive phenotype.
Characteristics: In this cell line Uba1 is heat-inactivatable (PubMed=10848989).
Transformant: ChEBI; CHEBI:23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Derived from site: In situ; Embryo; UBERON=UBERON_0000922.
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: BALB/c.
Hierarchy Parent: CVCL_0184 (BALB/3T3 clone A31)
Sex of cell Sex unspecified
Age at sampling 14-17FD
Category Transformed cell line
Publications

PubMed=6933701; DOI=10.1007/BF01538640
Jha K.K., Siniscalco M., Ozer H.L.
Temperature-sensitive mutants of Balb/3T3 cells. III. Hybrids between ts2 and other mouse mutant cells affected in DNA synthesis and correction of ts2 defect by human X chromosome.
Somatic Cell Genet. 6:603-614(1980)

PubMed=6092941; DOI=10.1128/mcb.4.9.1815-1822.1984
Zeng G.-C., Donegan J.J., Ozer H.L., Hand R.
Characterization of a ts mutant of BALB/3T3 cells and correction of the defect by in vitro addition of extracts from wild-type cells.
Mol. Cell. Biol. 4:1815-1822(1984)

PubMed=2995094; DOI=10.1016/0014-4827(85)90247-2
Zeng G.-C., Ozer H.L., Hand R.
Further characterization of the phenotype of ts20, a DNAts mutant of BALB/3T3 cells.
Exp. Cell Res. 160:184-196(1985)

PubMed=8114731; DOI=10.1128/mcb.14.3.1997-2003.1994
Chowdary D.R., Dermody J.J., Jha K.K., Ozer H.L.
Accumulation of p53 in a mutant cell line defective in the ubiquitin pathway.
Mol. Cell. Biol. 14:1997-2003(1994)

PubMed=10848989; DOI=10.1046/j.1432-1327.2000.01404.x
Salvat C., Acquaviva C., Scheffner M., Robbins I., Piechaczyk M., Jariel-Encontre I.
Molecular characterization of the thermosensitive E1 ubiquitin-activating enzyme cell mutant A31N-ts20. Requirements upon different levels of E1 for the ubiquitination/degradation of the various protein substrates in vivo.
Eur. J. Biochem. 267:3712-3722(2000)

Cross-references
Anatomy/cell type resources BTO; BTO:0004582
BTO; BTO:0005283
Encyclopedic resources Wikidata; Q54606748
Entry history
Entry creation06-Jun-2012
Last entry update29-Jun-2023
Version number14