ID   BALB/3T3 clone A31 ts20
AC   CVCL_B385
AS   CVCL_D594
SY   ts20; Ts20; TS-20; A31N-ts20; A31N ts20; A31Nts20
DR   BTO; BTO:0004582
DR   BTO; BTO:0005283
DR   Wikidata; Q54606748
RX   PubMed=2995094;
RX   PubMed=6092941;
RX   PubMed=6933701;
RX   PubMed=8114731;
RX   PubMed=10848989;
CC   Characteristics: Temperature sensitive phenotype.
CC   Characteristics: In this cell line Uba1 is heat-inactivatable (PubMed=10848989).
CC   Transformant: ChEBI; CHEBI:23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
CC   Derived from site: In situ; Embryo; UBERON=UBERON_0000922.
CC   Cell type: Fibroblast; CL=CL_0000057.
CC   Breed/subspecies: BALB/c.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_0184 ! BALB/3T3 clone A31
SX   Sex unspecified
AG   14-17FD
CA   Transformed cell line
DT   Created: 06-06-12; Last updated: 29-06-23; Version: 14
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RX   PubMed=2995094; DOI=10.1016/0014-4827(85)90247-2;
RA   Zeng G.-C., Ozer H.L., Hand R.;
RT   "Further characterization of the phenotype of ts20, a DNAts mutant of
RT   BALB/3T3 cells.";
RL   Exp. Cell Res. 160:184-196(1985).
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RX   PubMed=6092941; DOI=10.1128/mcb.4.9.1815-1822.1984;
RA   Zeng G.-C., Donegan J.J., Ozer H.L., Hand R.;
RT   "Characterization of a ts mutant of BALB/3T3 cells and correction of
RT   the defect by in vitro addition of extracts from wild-type cells.";
RL   Mol. Cell. Biol. 4:1815-1822(1984).
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RX   PubMed=6933701; DOI=10.1007/BF01538640;
RA   Jha K.K., Siniscalco M., Ozer H.L.;
RT   "Temperature-sensitive mutants of Balb/3T3 cells. III. Hybrids between
RT   ts2 and other mouse mutant cells affected in DNA synthesis and
RT   correction of ts2 defect by human X chromosome.";
RL   Somatic Cell Genet. 6:603-614(1980).
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RX   PubMed=8114731; DOI=10.1128/mcb.14.3.1997-2003.1994;
RA   Chowdary D.R., Dermody J.J., Jha K.K., Ozer H.L.;
RT   "Accumulation of p53 in a mutant cell line defective in the ubiquitin
RT   pathway.";
RL   Mol. Cell. Biol. 14:1997-2003(1994).
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RX   PubMed=10848989; DOI=10.1046/j.1432-1327.2000.01404.x;
RA   Salvat C., Acquaviva C., Scheffner M., Robbins I., Piechaczyk M.,
RA   Jariel-Encontre I.;
RT   "Molecular characterization of the thermosensitive E1
RT   ubiquitin-activating enzyme cell mutant A31N-ts20. Requirements upon
RT   different levels of E1 for the ubiquitination/degradation of the
RT   various protein substrates in vivo.";
RL   Eur. J. Biochem. 267:3712-3722(2000).
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