Cellosaurus RAW-Dual KO-TLR4 (CVCL_A7ZL)
| Cell line name | RAW-Dual KO-TLR4 |
|---|---|
| Accession | CVCL_A7ZL |
| Resource Identification Initiative | To cite this cell line use: RAW-Dual KO-TLR4 (RRID:CVCL_A7ZL) |
| Comments | Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. ALPP expression depends on the activation of the endogenous MIP-2/CXCL2 promoter as the Cxcl2 gene has been replaced by ALPP. The Lucia luciferase reporter gene is under the control of an ISG54 minimal promoter in conjunction with 5 IFN-stimulated response elements (ISRE). Knockout cell: Method=Not specified; MGI; MGI:1340094; Cxcl2. Knockout cell: Method=Not specified; MGI; MGI:96824; Tlr4. Genetic integration: Method=Transfection/transduction; Gene=HGNC; 439; ALPP. Genetic integration: Method=Transfection/transduction; Gene=Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Transformant: NCBI_TaxID; 11788; Abelson mouse leukemia virus (AbMuLV). Derived from site: In situ; Ascites; UBERON=UBERON_0007795. Cell type: Macrophage; CL=CL_0000235. |
| Disease | Mouse leukemia (NCIt: C21604) |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: BALB/c. |
| Hierarchy | Parent: CVCL_A7ZK (RAW-Dual) |
| Sex of cell | Male |
| Age at sampling | Adult |
| Category | Cancer cell line |
| Web pages | https://www.invivogen.com/raw-dual-ko-tlr4 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q107116717 |
| Entry history | |
| Entry creation | 20-May-2021 |
| Last entry update | 10-Sep-2024 |
| Version number | 8 |