Cellosaurus RAW-Dual (CVCL_A7ZK)
| Cell line name | RAW-Dual | |
|---|---|---|
| Synonyms | RAW IRF-Lucia/KI-[MIP-2]SEAP | |
| Accession | CVCL_A7ZK | |
| Resource Identification Initiative | To cite this cell line use: RAW-Dual (RRID:CVCL_A7ZK) | |
| Comments | Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. ALPP expression depends on the activation of the endogenous MIP-2/CXCL2 promoter as the Cxcl2 gene has been replaced by ALPP. The Lucia luciferase reporter gene is under the control of an ISG54 minimal promoter in conjunction with 5 IFN-stimulated response elements (ISRE). Knockout cell: Method=Not specified; MGI; MGI:1340094; Cxcl2. Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:439; ALPP. Genetic integration: Method=Transfection/transduction; Gene=Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Transformant: NCBI_TaxID; 11788; Abelson mouse leukemia virus (AbMuLV). Derived from site: In situ; Ascites; UBERON=UBERON_0007795. Cell type: Macrophage; CL=CL_0000235. | |
| Disease | Mouse leukemia (NCIt: C21604) | |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: BALB/c. | |
| Hierarchy | Parent: CVCL_0493 (RAW 264.7) Children:
| |
| Sex of cell | Male | |
| Age at sampling | Adult | |
| Category | Cancer cell line | |
| Web pages | https://www.invivogen.com/raw-dual | |
| Cross-references | ||
| Encyclopedic resources | Wikidata; Q107116716 | |
| Entry history | ||
| Entry creation | 20-May-2021 | |
| Last entry update | 19-Dec-2024 | |
| Version number | 9 | |