ID   RAW-Dual KO-TLR4
AC   CVCL_A7ZL
DR   Wikidata; Q107116717
WW   https://www.invivogen.com/raw-dual-ko-tlr4
CC   Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. ALPP expression depends on the activation of the endogenous MIP-2/CXCL2 promoter as the Cxcl2 gene has been replaced by ALPP. The Lucia luciferase reporter gene is under the control of an ISG54 minimal promoter in conjunction with 5 IFN-stimulated response elements (ISRE).
CC   Knockout cell: Method=Not specified; MGI; MGI:1340094; Cxcl2.
CC   Knockout cell: Method=Not specified; MGI; MGI:96824; Tlr4.
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; 439; ALPP.
CC   Genetic integration: Method=Transfection/transduction; Gene=Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Transformant: NCBI_TaxID; 11788; Abelson mouse leukemia virus (AbMuLV).
CC   Derived from site: In situ; Ascites; UBERON=UBERON_0007795.
CC   Cell type: Macrophage; CL=CL_0000235.
CC   Breed/subspecies: BALB/c.
DI   NCIt; C21604; Mouse leukemia
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_A7ZK ! RAW-Dual
SX   Male
AG   Adult
CA   Cancer cell line
DT   Created: 20-05-21; Last updated: 10-09-24; Version: 8
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