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Cellosaurus NIKS (CVCL_A1LW)

[Text version]
Cell line name NIKS
Synonyms BC1-Ep/SL; BC-1-Ep/SL; Normal Immortal KeratinocyteS
Accession CVCL_A1LW
Resource Identification Initiative To cite this cell line use: NIKS (RRID:CVCL_A1LW)
Comments Group: Patented cell line.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-12191.
Biotechnology: Can be used to construct a living bioengineered skin substitute (StrataGraft) intended to provide immediate wound coverage and promote wound healaling through sustained expression by living cells of wound healing factors (PubMed=24527287).
Biotechnology: Can be used to construct a human skin model (Stratatest) that can be used to assess tissue damage caused by chemical or physical agents and for the identification and characterization of agents formulated to mitigate this damage (PubMed=20688150).
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Miscellaneous: STR profile from personal communication of Lee, Denis L.
Derived from site: In situ; Foreskin, skin, epidermis; UBERON=UBERON_0001471+UBERON_0001003.
Cell type: Keratinocyte; CL=CL_0000312.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_B0UM (NIKS-16)CVCL_B0UN (NIKS-16deltaE7)CVCL_B0UP (NIKS-18)
Sex of cell Male
Age at sampling <1M
Category Spontaneously immortalized cell line
STR profile Source(s): Direct_author_submission

Markers:
AmelogeninX,Y
CSF1PO10
D3S135815,16
D5S81811,12
D7S82010,12
D8S117911,13
D13S31710,13
D16S53911,13
D18S5114,17
D21S1131,31.2
FGA20,24
Penta D12,17
Penta E7,17
TH019,10
TPOX8
vWA14,17

Run an STR similarity search on this cell line
Publications

PubMed=10502513; DOI=10.1006/viro.1999.9868
Flores E.R., Allen-Hoffmann B.L., Lee D.L., Sattler C.A., Lambert P.F.
Establishment of the human papillomavirus type 16 (HPV-16) life cycle in an immortalized human foreskin keratinocyte cell line.
Virology 262:344-354(1999)

PubMed=10692102; DOI=10.1046/j.1523-1747.2000.00869.x
Allen-Hoffmann B.L., Schlosser S.J., Rasmussen C.A., Sattler C.A., Meisner L.F., O'Connor S.L.
Normal growth and differentiation in a spontaneously immortalized near-diploid human keratinocyte cell line, NIKS.
J. Invest. Dermatol. 114:444-455(2000)

Patent=US6884595
Allen-Hoffmann B.L., Schlosser S.J.
Immortalized human keratinocyte cell line.
Patent number US6884595, 26-Apr-2005

PubMed=20688150; DOI=10.1016/j.tiv.2010.07.027
Rasmussen C.A., Gratz K., Liebel F., Southall M., Garay M., Bhattacharyya S., Simon N., Vander Zanden M., Van Winkle K., Pirnstill J., Pirnstill S., Comer A., Allen-Hoffmann B.L.
The StrataTest(R) human skin model, a consistent in vitro alternative for toxicological testing.
Toxicol. In Vitro 24:2021-2029(2010)

PubMed=24527287; DOI=10.1089/wound.2011.0343; PMCID=PMC3623596
Schurr M.J., Foster K.N., Lokuta M.A., Rasmussen C.A., Thomas-Virnig C.L., Faucher L.D., Caruso D.M., Allen-Hoffmann B.L.
Clinical evaluation of NIKS-based bioengineered skin substitute tissue in complex skin defects: phase I/IIa clinical trial results.
Adv. Wound Care (New Rochelle) 1:95-103(2012)

PubMed=23899513; DOI=10.1016/j.jss.2013.06.046
Roy M., King T.W.
Epidermal growth factor regulates NIKS keratinocyte proliferation through Notch signaling.
J. Surg. Res. 185:6-11(2013)

PubMed=28623356; DOI=10.1038/s41598-017-03295-7; PMCID=PMC5473897
Cicchini L., Blumhagen R.Z., Westrich J.A., Myers M.E., Warren C.J., Siska C., Raben D., Kechris K.J., Pyeon D.
High-risk human papillomavirus E7 alters host DNA methylome and represses HLA-E expression in human keratinocytes.
Sci. Rep. 7:3633-3633(2017)

PubMed=29021401; DOI=10.1128/JVI.01001-17; PMCID=PMC5709591
Klymenko T., Gu Q., Herbert I., Stevenson A., Iliev V., Watkins G., Pollock C.S., Bhatia R., Cuschieri K., Herzyk P., Gatherer D., Graham S.V.
RNA-Seq analysis of differentiated keratinocytes reveals a massive response to late events during human papillomavirus 16 infection, including loss of epithelial barrier function.
J. Virol. 91:e01001.17.1-e01001.17.17(2017)

Cross-references
Encyclopedic resources Wikidata; Q102114626
Gene expression databases GEO; GSM2197772
GEO; GSM2197773
GEO; GSM2197774
GEO; GSM3763043
Entry history
Entry creation29-Oct-2020
Last entry update10-Sep-2024
Version number7