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Cellosaurus U373-MAGI (CVCL_1F84)

[Text version]
Cell line name U373-MAGI
Synonyms U373-CD4-LTR/beta-gal; U373-Multinuclear Activation of a Galactosidase Indicator
Accession CVCL_1F84
Resource Identification Initiative To cite this cell line use: U373-MAGI (RRID:CVCL_1F84)
Comments Population: Caucasian.
Characteristics: Transfected with a construct pEQ447 containing the HIV-1 LTR fused to a lacZ reporter gene that contains a MPKKKRK N-terminal sequence directing it to the nucleus (PubMed=7690415).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1678; CD4.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: In situ; Brain, parietal lobe; UBERON=UBERON_0001872.
Disease Astrocytoma (NCIt: C60781)
Astrocytoma (ORDO: Orphanet_94)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_E8AN (U373-CD4)
Children:
CVCL_1F85 (U373-MAGI-CCR5)CVCL_1F86 (U373-MAGI-CXCR4)
Sex of cell Male
Age at sampling 75Y
Category Cancer cell line
Publications

PubMed=7690415; DOI=10.1128/JVI.67.10.5939-5947.1993; PMCID=PMC238014
Harrington R.D., Geballe A.P.
Cofactor requirement for human immunodeficiency virus type 1 entry into a CD4-expressing human cell line.
J. Virol. 67:5939-5947(1993)

PubMed=9201229; DOI=10.1006/viro.1997.8606
Vodicka M.A., Goh W.C., Wu L.I., Rogel M.E., Bartz S.R., Schweickart V.L., Raport C.J., Emerman M.
Indicator cell lines for detection of primary strains of human and simian immunodeficiency viruses.
Virology 233:193-198(1997)

Cross-references
Cell line collections (Providers) BEI_Resources; ARP-3595
Chemistry resources ChEMBL-Cells; CHEMBL3886081
PubChem_Cell_line; CVCL_1F84
Encyclopedic resources Wikidata; Q54973736
Entry history
Entry creation08-Jul-2015
Last entry update10-Apr-2025
Version number14