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Cellosaurus U373-MAGI-CCR5 (CVCL_1F85)

[Text version]
Cell line name U373-MAGI-CCR5
Synonyms U373-MAGI-CCR5E
Accession CVCL_1F85
Resource Identification Initiative To cite this cell line use: U373-MAGI-CCR5 (RRID:CVCL_1F85)
Comments Population: Caucasian.
Characteristics: Transfected with a construct pEQ447 containing the HIV-1 LTR fused to a lacZ reporter gene that contains a MPKKKRK N-terminal sequence directing it to the nucleus (from a parent cell line).
Virology: Can be used to detect primary isolates of HIV-1, HIV-2 and SIV (PubMed=9201229).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1606; CCR5.
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1678; CD4.
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: In situ; Brain, parietal lobe; UBERON=UBERON_0001872.
Disease Astrocytoma (NCIt: C60781)
Astrocytoma (ORDO: Orphanet_94)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1F84 (U373-MAGI)
Sex of cell Male
Age at sampling 75Y
Category Cancer cell line
Publications

PubMed=9201229; DOI=10.1006/viro.1997.8606
Vodicka M.A., Goh W.C., Wu L.I., Rogel M.E., Bartz S.R., Schweickart V.L., Raport C.J., Emerman M.
Indicator cell lines for detection of primary strains of human and simian immunodeficiency viruses.
Virology 233:193-198(1997)

Cross-references
Cell line collections (Providers) BEI_Resources; ARP-3597
Encyclopedic resources Wikidata; Q54973738
Entry history
Entry creation08-Jul-2015
Last entry update10-Apr-2025
Version number12