Cellosaurus cell line B16-F10 (CVCL

Cellosaurus B16-F10 (CVCL_0159)

[Text version]

Cell line name

B16-F10

Synonyms

B16/F10; B16 F10; B16F10; B16 melanoma F10

Accession

CVCL_0159

Resource Identification Initiative

To cite this cell line use: B16-F10 (RRID:CVCL_0159)

Comments

Characteristics: Obtained from the pulmonary melanoma module of the 10th serial passage of the B16-F0 parent cell line in C57BL/6 mice.
Characteristics: Has a high metastatic potential.
Doubling time: 17 hours (PubMed=1109790); 17.2 hours (PubMed=23890195).
Omics: CRISPR phenotypic screen.
Omics: HLA class I peptidome analysis by proteomics.
Omics: SNP array analysis.
Derived from site: In situ; Skin; UBERON=UBERON_0002097.

Disease

Mouse melanoma (NCIt: C21790)

Species of origin

Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6.

Hierarchy

Parent: CVCL_UJ41 (B16-F5)
Children:

CVCL_0157 (B16-BL6)	CVCL_D0P3 (B16-F10 B2m-KO)	CVCL_E3VN (B16-F10 Pbrm1 KO 1)
CVCL_E3VP (B16-F10 Pbrm1 KO 2)	CVCL_XB96 (B16-F10-EGFP)	CVCL_U240 (B16-F10-Lr6)
CVCL_C8XU (B16-F10-Luc)	CVCL_4Y00 (B16-F10-Luc 1-#3)	CVCL_5J39 (B16-F10-luc-G5)
CVCL_A4CJ (B16-F10-Luc2 [ATCC])	CVCL_5J17 (B16-F10-luc2 [Caliper])	CVCL_XB95 (B16-F10-mCherry)
CVCL_F942 (B16-F10-Nex2)	CVCL_C3Q0 (B16-F10-Nex3)	CVCL_5I87 (B16-F10-Red-FLuc)
CVCL_XB97 (B16-F10-tdT)	CVCL_E3VQ (B16-F10.9)	CVCL_JG40 (B16-F10/CMV-Luc#2)
CVCL_JG94 (B16-F10/EF1a-Luc#1)	CVCL_IJ12 (B16-FLT3L)	CVCL_B7NS (B16/F10-Luc-RFP)
CVCL_VA21 (B16F10-B7H6)	CVCL_QZ86 (B16F10-eGFP-Puro)	CVCL_D5KB (B16F10-Fluc-hNIS)
CVCL_QZ87 (B16F10-Fluc-Neo/eGFP-Puro)	CVCL_QZ88 (B16F10-Fluc-Neo/iRFP-Puro)	CVCL_QZ89 (B16F10-Fluc-Neo/mNIS-Puro)
CVCL_QZ90 (B16F10-Fluc-Puro)	CVCL_QZ91 (B16F10-iRFP-Puro)	CVCL_QZ92 (B16F10-mNIS (monoclonal))
CVCL_QZ93 (B16F10-mNIS-Neo/eGFP-Puro)	CVCL_QZ94 (B16F10-mNIS-Neo/iRFP-Puro)	CVCL_QZ95 (B16F10-mNIS-Puro)
CVCL_L292 (B16F10/mGM)	CVCL_XH27 (CDDP-R B16F10)	CVCL_D6AW (HyCyte B16-F10 KO-mBtnl2)
CVCL_D6AX (HyCyte B16-F10 KO-mFgl2)	CVCL_E1J9 (HyCyte B16-F10 KO-mPkm)	CVCL_E1JA (HyCyte B16-F10 KO-mRipk1)
CVCL_E1JB (HyCyte B16-F10 KO-mTmem176b)	CVCL_E3GU (HyCyte B16-F10-Luc)	CVCL_E3GV (HyCyte B16-F10-Luc-GFP)
CVCL_YJ76 (LINTERNA B16-F10)	CVCL_D8E2 (Ubigene B16-F10 Acad10 KO)	CVCL_D8E3 (Ubigene B16-F10 Art1 KO)
CVCL_D8E4 (Ubigene B16-F10 Cstf2 KO)	CVCL_D8E5 (Ubigene B16-F10 Cyp11a1 KO)	CVCL_D8E6 (Ubigene B16-F10 Egfr KO)
CVCL_D8E7 (Ubigene B16-F10 F3 KO)	CVCL_D8E8 (Ubigene B16-F10 Fas KO)	CVCL_D8E9 (Ubigene B16-F10 Glp1r KO)
CVCL_D8EA (Ubigene B16-F10 Klf12 KO)	CVCL_D8EB (Ubigene B16-F10 Msh2 KO)	CVCL_D8EC (Ubigene B16-F10 Notch3 KO)
CVCL_D8ED (Ubigene B16-F10 Spp1 KO)	CVCL_D8EE (Ubigene B16-F10 Tgfb3 KO)	CVCL_D8EF (Ubigene B16-F10 Ttc14 KO)
CVCL_D8EG (Ubigene B16-F10 Vtcn1 KO)	CVCL_D8EH (Ubigene B16-F10 Ythdf1 KO)	CVCL_YJ65 (VAMPIRO B16-F10)

Sex of cell

Male

Category

Cancer cell line

STR profile

Source(s): Ubigene=YC-A011

Markers:

Mouse STR 4-2	20.3,21.3
Mouse STR 5-5	16,20
Mouse STR 6-4	18,19
Mouse STR 6-7	15
Mouse STR 12-1	17,18
Mouse STR 15-3	22.3,23.3
Mouse STR 18-3	15,16
Mouse STR X-1	28

Web pages

https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/b/cell-lines-detail-1.html

Publications

PubMed=1109790
Fidler I.J.
Biological behavior of malignant melanoma cells correlated to their survival in vivo.
Cancer Res. 35:218-224(1975)

PubMed=210294; DOI=10.1093/jnci/61.2.523
Niles R.M., Makarski J.S.
Control of melanogenesis in mouse melanoma cells of varying metastatic potential.
J. Natl. Cancer Inst. 61:523-526(1978)

PubMed=6539703; DOI=10.1016/0277-5379(84)90015-4
Weinreb A., Travo P.
Discrimination between human melanoma cell lines by fluorescence anisotropy.
Eur. J. Cancer Clin. Oncol. 20:673-677(1984)

PubMed=11833741; DOI=10.1016/S0024-3205(01)01454-0
Nakamura K., Yoshikawa N., Yamaguchi Y., Kagota S., Shinozuka K., Kunitomo M.
Characterization of mouse melanoma cell lines by their mortal malignancy using an experimental metastatic model.
Life Sci. 70:791-798(2002)

PubMed=23890195; DOI=10.1186/1475-2867-13-75; PMCID=PMC3750233
Danciu C., Falamas A., Dehelean C., Soica C., Radeke H., Barbu-Tudoran L., Bojin F., Cinta Pinzaru S., Munteanu M.F.
A characterization of four B16 murine melanoma cell sublines molecular fingerprint and proliferation behavior.
Cancer Cell Int. 13:75.1-75.12(2013)

PubMed=25277546; DOI=10.1186/1471-2164-15-847; PMCID=PMC4198738
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. 3rd, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

PubMed=30084848; DOI=10.1038/sdata.2018.157; PMCID=PMC6080492
Schuster H., Shao W.-G., Weiss T., Pedrioli P.G.A., Roth P., Weller M., Campbell D.S., Deutsch E.W., Moritz R.L., Planz O., Rammensee H.-G., Aebersold R., Caron E.
A tissue-based draft map of the murine MHC class I immunopeptidome.
Sci. Data 5:180157-180157(2018)

Cross-references

Cell line collections (Providers)