Cellosaurus B16F10-Fluc-Neo/iRFP-Puro (CVCL_QZ88)
| Cell line name | B16F10-Fluc-Neo/iRFP-Puro |
|---|---|
| Accession | CVCL_QZ88 |
| Resource Identification Initiative | To cite this cell line use: B16F10-Fluc-Neo/iRFP-Puro (RRID:CVCL_QZ88) |
| Comments | Characteristics: Reporter cell line, transfected with both the LV-Fluc-P2A-Neo and LV-iRFP-P2A-Puro vectors. LV-Fluc-P2A-Neo encodes for luciferase linked via a P2A cleavage peptide to the neo gene and under control of the spleen focus-forming virus (SFFV) promoter. LV-iRFP-P2A-Puro encodes for iRFP linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (Imanis=CL110). Genetic integration: Method=Transfection; Gene=FPbase; DGXFA; iRFP (Note=iRFP713, near infra-red fluorescent protein from R.palustris). Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase. Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Skin; UBERON=UBERON_0002097. |
| Disease | Mouse melanoma (NCIt: C21790) |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6. |
| Hierarchy | Parent: CVCL_0159 (B16-F10) |
| Sex of cell | Male |
| Category | Cancer cell line |
| Cross-references | |
| Cell line collections (Providers) | Imanis; CL110 |
| Encyclopedic resources | Wikidata; Q54752466 |
| Entry history | |
| Entry creation | 05-Mar-2018 |
| Last entry update | 10-Sep-2024 |
| Version number | 9 |