| Abstract |
Background and aims: To identify and isolation of viruses a susceptible
cell is need which can be primary or line. The use of primary cells is
laborious, time consuming and expensive, while the use of cell lines is
much easier and faster. IRKHBK cell line was originated from primary cells
of bovine kidney by continuous passage. The aim of this study was to
introduce the IRKHBK cell line for isolation, propagation and diagnosis of
animal viruses.
Material and methods: Samples of the infected animals, such as the lymph
nodes, tonsils, lungs and blood were used for virus isolation. Primary BK
cell and IRKHBK cell line cultures were inoculated with the prepared
samples simultaneously under the same conditions. Identification of the
isolated viruses was carried out with virus neutralization test using
standard antibodies.
Results: Bovine Herpes Virus-1, Parainfluenza-3, Bovine viral diarrhea,
and Peste des Petits Ruminants Virus were easily isolated and propagated
in IRKHBK cells. The viral titer was higher in IRKHBK cell line compared
to the primary BK cell. The IRKHBK cell line also showed considerable
susceptibility in serological tests.
Conclusion: The results show that IRKHBK cell line can be used for virus
isolation, detection and propagation. This cell line can be used for
animal and human viruses research studies and also other pathogens that
are able to grow in the cell culture.
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