| Publication number |
CLPUB00584 |
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| Authors |
Motoyama T., Hojo H., Suzuki T., Oboshi S. |
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| Title |
Evaluation of the regrowth assay method as an in vitro drug sensitivity test and its application to cultured human gastric cancer cell lines. |
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| Citation |
Acta Med. Biol. 27:49-63(1979) |
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| Abstract |
There are several methods for assay of in vitro drug sensitivity of
cultured cancer cells. The present study first deals with the validity of
two representative methods of in vitro sensitivity tests: the growth
inhibition method expressed by a sensitivity index of IC50 (50% inhibition
concentration) and the regrowth assay method expressed by an index of LD90
(90% lethal dose). The regrowth assay method has been confirmed to be
highly reproducible and the sensitivity index of LD90 is not influenced by
the inoculation size of test cells, the day judged, and the population
doubling time of test cells. Therefore, the drug sensitivity among cell
lines could be evaluated by comparing their indices. On the other hand,
the IC50 is not reproducible and influenced by the 3 factors above
mentioned. Therefore, it might be concluded that IC50 is not suitable as
an index of sensitivity of cancer cells to anticancer drugs at least in
terms of cytocidal activity. Secondly, the result of sensitivity tests of
9 human stomach cancer cell lines to mitomycin C measured by regrowth
assay is reported. In general, cell lines derived from poorly
differentiated adenocarcinoma are more sensitive to the test drug than
those from well differentiated tubular adenocarcinoma.
|
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| Cell lines |
CVCL_0371; KATO III CVCL_A1JM; MK2 CVCL_1415; MKN1 CVCL_1417; MKN7 CVCL_1416; MKN28 CVCL_0434; MKN45 CVCL_2791; MKN74 CVCL_9815; Okajima CVCL_1687; SCH |
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