| Publication number |
CLPUB00542 |
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| Authors |
Miyoshi I., Taguchi H., Kubonishi I., Yoshimoto S., Ohtsuki Y., Shiraishi Y., Akagi T. |
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| Title |
Type C virus-producing cell lines derived from adult T cell leukemia. |
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| Citation |
Gann Monogr. Cancer Res. 28:219-228(1982) |
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| Abstract |
We have established four unique cell lines, MT-1 to MT-4, by co-
cultivation of adult T-cell leukemia (ATL) cells and human cord blood
lymphocytes. All four cell lines possessed T-cell properties and lacked
Epstein-Barr virus nuclear antigen. Chromosome analysis revealed MT-1 to
be of ATL cell origin whereas at least two others, MT-2 and MT-3, were of
cord lymphocyte origin. All these cell lines were found to be persistently
infected with type C virus particles (ATLV) and positive for ATL-
associated antigens (ATLA) which reacted specifically with sera from ATL
patients. MT-2 was the highest producer of ATLV and MT-1 the lowest. The
three cell lines tested, MT-1 to MT-3, were tumorigenic when transplanted
into immunosuppressed newborn hamsters. Only the tumors produced with MT-3
were examined for virus and they were positive for ATLV and ATLA. Lymphoid
cell lines with human chromosomes were readily recultured from MT-3-
induced tumors.
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| Cell lines |
CVCL_M518; MT-1 [Human leukemia] CVCL_2631; MT-2 CVCL_M517; MT-3 [Human leukocyte] CVCL_2632; MT-4 |
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