| Abstract |
Some special properties of a human promonocyte cell line, HL-CZ, were
characterized after long-term cloning and subcloning in vitro. About 90%
of the cells was detected with CD34-like marker, and 37% of them shown
CD11b positive. Meanwhile, 17% of them had phagocytosis activity when
evaluated by flow cytometry. It was also found that 30-69.3% of the cells
were interleukin 6 positive, 17.8-19.3% and 28-73% were interleukin 1-beta
and tumor necrosis factor alpha (TNF-alpha) positive, respectively, when
detected by indirect immunofluorecence. In the cytochemical studies, none
of them had peroxidase or nonspecific esterase activities, whereas
granular type of periodic acid Schiff reaction was seen in small
population of the cells. An interesting phenomenon was that the
heterogeneity was found in every subclone of long-term culture. Some huge
cells were always present after 2-3 days of culture. When co-cultured with
hepatitis C patient serum, the percentage of huge cells increased and an
autofusion behavior was observed. This implied that some unusual
mechanisms existed in this cell population.
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