Due to maintenance work, this service will be unavailable from
Mon Nov 11 17:30 until
Tue Nov 12 09:00
CET.
Apologies for the inconvenience.
Cellosaurus publication CLPUB00508
| Publication number | CLPUB00508 |
|---|---|
| Authors | Chen D., Chen H., Feng J.Q., Windle J.J., Koop B.A., Harris M.A., Bonewald L.F., Boyce B.F., Wozney J.M., Mundy G.R., Harris S.E. |
| Title | Osteoblastic cell lines derived from a transgenic mouse containing the osteocalcin promoter driving SV40 T-antigen. |
| Citation | Mol. Cell. Differ. 3:193-212(1995) |
| Abstract | The object of this study was to develop new murine osteoblast-like cell lines for studying bone cell differentiation. In an attempt to develop cell lines representing a specific stage in osteoblast differentiation, we utilized transgenic mice. Immortalized osteoblastic cells were isolated and cloned from the calvaria of a transgenic mouse containing a 2.6-kb fragment of the rat osteocalcin promoter driving the expression of SV40 large T antigen. Two clonal cell lines, OCT-1 and OCT-2, were characterized. T-antigen expression by these two cell lines was confirmed using T-antigen antibody. Cell lines were tested for their responsiveness to parathyroid hormone (PTH), prostaglandin B2 (PGE2), 1,25- dihydroxyvitamin D3 (1,25D3), bone morphogenetic protein-2 (BMP-2), transforming growth factor beta (TGF-beta), and retinoic acid. Their capacity to produce mineralized bone nodules and to express type I collagen, alkaline phosphatase (ALP), and osteocalcin at the mRNA level was also evaluated. Osteocalcin expression was found to be very low: OCT-1 and OCT-2 cells injected into nude mice subcutaneously over the surface of calvaria caused osteosarcomas in 10 and 6 weeks, respectively. Significant new bone formation was associated with the tumors. OCT-1 and OCT-2 cells have different response profiles to BMP-2, retinoic acid, PTH, and PGE2. These results demonstrate that OCT-1 and OCT-2 are cells representative of different stages of osteoblast differentiation. They have low levels of osteocalcin expression and may offer a tool to study the role of osteocalcin in bone formation and mineralization. |
| Cell lines | CVCL_WN86; OCT-1 CVCL_WN87; OCT-2 |