| Publication number |
CLPUB00368 |
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| Authors |
Polanowski F.P., Gaffney E.V., Burke R.E. |
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| Title |
HBL-100, a cell line established from human breast milk. |
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| Citation |
In Vitro 12:328-328(1976) |
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| Web pages |
https://link.springer.com/article/10.1007/BF02801988 |
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| Abstract |
The purpose of this study was to characterize a line of cells established
from a normal source of human mammary epithelium. Human milk samples
collected from nursing mothers during the first week postpartum contain
both colony forming epithelium, desquamated from ducts and alveoli; and
foam cells responsible for the synthesis of milk products. These cells can
be maintained in monolayer culture for several weeks. Two samples of milk
from the 300 patients initially surveyed contained cells capable of
continuous growth in vitro. One cell line, HBL-100, has been transferred
through 90 weekly passages. These cells do not form tumors in nude mice
but will grow in soft agar. Of 60 metaphases examined during passage 8 the
number of chromosomes per cell varied from 58 to 73 with a hypertriploid
mean of 66. The 80 of 83 metaphases scanned during passage 50 had
chromosome numbers in the low 60 range. No HeLa marker chromosomes were
found, and cells exhibited a B-type G6PD electrophoretic pattern. Most of
the metaphases had one or more minute chromosomes. Electron micrographs
revealed the presence of desmosomes. Although radioimmunoassay for alpha-
lactalbumin was negative, the cells do synthesize a small amount of
lactose. HBL-100 cells respond to prolactin and/or estrogen by
synthesizing increased amounts of isoelectrically precipitable casein.
These observations demonstrated that this cell line may classify as: (1)
epithelial, originating from a normal source; (2) transformed but not
malignant; and (3) mammary inferred from their capacity to respond to
lactogenic hormones.
|
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| Cell lines |
CVCL_4362; HBL-100 |
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