| Publication number |
CLPUB00339 |
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| Authors |
Mayyasi S.A., Traul K.A., Garon C.E., Wright B. |
|---|
| Title |
Antigenics characteristics of Rauscher virus cultivated in human embryonic kidney cells. |
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| Citation |
Proc. Am. Assoc. Cancer Res. 11:54.211-54.211(1970) |
|---|
| Abstract |
The antigenicity of Rauscher murine leukemia virus (RV) grown in human
embryonic kidney cells and cellular material extracted from infected cells
were compared to the same type of antigens extracted from mouse infected
cells. The purity of the infected human line was verified by karyotype
analysis and by the cell mixed agglutination test. Group specific rat
antisera reacted in a similar manner with mouse and human derived infected
cells by the complement fixation test. Similar findings were revealed by
immunofluorescence test where no difference could be detected between the
human or mouse infected cells. The virus agglutination test revealed
differences between both viruses. Rabbit anti-mouse RV serum agglutinated
the homotypic but not the heterotypic virus. Group specific rat antisera
failed to agglutinate both types of virus. Gel diffusion studies conducted
with intact and ether treated viruses showed lines of identity with the
nucleoids of the two viruses. The mouse grown virus showed a coat
component which was absent from the human grown RV. The results presented
here indicate that the RV grown in human cells acquires new immunological
characteristics as a result of the new human envelope acquired during
virus formation.
|
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| Cell lines |
CVCL_M624; HEK |
|---|
