| Abstract |
A cell line designated HIOAnu was established from the human ovarian
endometrioid adenocarcinoma. On Feb. 23, 1999, a Virchow's lymph node
tumor (metastatic regions of ovarian carcinoma) was removed from a 49 year-
old Japanese woman and was placed into culture the next day. A portion of
tumor was transplanted subcutaneously into a BALB-c/nu nude mouse.
Histopathological diagnosis of the tumor indicated a endometrioid
adenocarcinoma, Primary culture of the tumor failed, while the transplant
into the nude mouse tumor was successful. The mouse graft was removed,
minced with a sharp pair of scissors, treated with 600 Pronase units/ml
dispase, and centrifuged. The tissues were placed in 6 cm plastic dishes
containing Ham's F12 medium (GIBCO), 100 units of penicillin and 100 jig
of streptomycin per ml, and 15% fetal calf serum; the final pH was 7.2-7.4.
The dishes were incubated at 37 Celsisus in a CO2 incubator supplied with
humidified 5% CO2 and 95% air. In the initiation of the primary culture,
an epithelial colony and fibroblastic cells appeared. Pure epithelial
cells were obtained by the isolation technique devised by Okumura. The
cell line has grown rapidly and serial passages were successively carried
out 42 times within 12 months. The cultured cells were spindle or
polygonal in shape, showing anaplastic pleomorphic features and
multilayering without contact inhibition. The population doubling time was
34 hours. The chromosomes showed a wide distribution of aneuploidy. The
mode was in the triploid range, and many marker chromosomes were observed.
The HIOAnu cells showed high transplantability into nude mice, produced an
endometrioid adenocarcinoma resembling the original tumor and a large
amount of CA 125 and TPA.
|
|---|
