| Abstract |
Three human cancer cell lines (KOC-4c, KOC-5c, KOC-7c) were newly
established from an untreated tumor tissue, ascitic fluid and pleural
effusion of patients with clear cell carcinoma of the ovary. These
cultured cell lines were transplantable into nude mice, and the pathologic
features of the transplants were similar to those of the original tumors.
Furthermore, KOC-4c was intraperitoneally transplantable to exhibit a
picture of peritoneal carcinomatosis, suggesting a feasible experimental
model for human ovarian cancer. There was remarkable difference between the
population doubling times of KOC-5c and KOC-7c, showing 97.9 hours for the
former and 45.9 hours for the latter. A more anaplastic histologic feature
of KOC-7c, mainly comprising hobnail cell patterns, might contribute the
difference of doubling time. Tumor markers, CA125 and SLX, were detectable
in the culture medium of KOC-5c cell line, as well as the serum of the
patient with original cancer. Although the original tumors were negatively
stained with mutant p53 protein, the gene protein was found in both KOC-5c
and KOC-7c. The results suggest that these established cell lines give
further merit to study this clinically intractable ovarian epithelial
cancer.
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