Due to maintenance work, this service will be unavailable from
Mon Nov 11 17:30 until
Tue Nov 12 09:00
CET.
Apologies for the inconvenience.
Cellosaurus publication CLPUB00060
| Publication number | CLPUB00060 |
|---|---|
| Authors | Gramatzki M., Burger R., Trautmann U., Marschalek R., Lorenz H., Hansen-Hagge T.E., Baum W., Bartram C.R., Gebhart E., Kalden J.R. |
| Title | Two new interleukin-6 dependent plasma cell lines carrying a chromosomal abnormality involving the IL-6 gene locus. |
| Citation | Blood 84 Suppl. 1:173a-173a(1994) |
| Abstract | Two plasma cell lines, INA-6 and JK-6, have been initiated and continuously cultured from two patients with malignant plasma cell diseases. Both cell lines are EBNA negative and show morphological and immunophenotypical features of plasma cells. INA-6 expresses the CD39 and CDw75 antigens, JK-6 is strongty positive with CD38 and CD39 antibodies. By flow cytometry they were non-reactive with Ia antibodies and B ceil reagents CD19, CD20, CD21, CD22, and CD24. While INA-6 cells are releasing kappa light chains only, JK-6 cells produce IgG kappa. Both cell lines could only be initiated with IL-6 supplemented medium and remained IL-6 responsive throughout continuous culture. INA-6 is strictly dependent on IL-6. No spontaneously secreted IL-6 was found nor could it be induced by IL-1beta /TNFalpha stimulation. Molecular analysis with RT-PCR revealed mRNA for the IL-6 receptor in both lines. No IL-6 mRNA was detectable in INA-6 cells, while in JK-6 minute amounts were observed. Cytogenetic analysis of both lines revealed, among other abnormalities, a deletion (7)(p13). lnterestingly, the 7p deletion affects the location ot the IL-6 gene. ln both cell lines, IL-6 dependent proliferation could be inhibited by IFNalpha. IFNalpha had growth regulatory effects only on JK-6: while high concentrations were inhibitory, low IFNalpha amounts were clearly stimulatory. A wide variety of other cytokines including GM-CSF and IL-11 did not have the capacity to influence proliferation. These plasma cell lines do not only allow to turther characterize regulatory events in plasma cell neoplasias but also provide tools to study therapeutic interventions. |
| Cell lines | CVCL_5209; INA-6 CVCL_M534; JK-6 |