| Publication number |
CLPUB00052 |
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| Authors |
Zipursky A., Vanek W., Grunberger T., Brown E.J., Wang H., Sutherland R., Yeo E.L., Cohen A., Christensen H., Freedman M.H. |
|---|
| Title |
A megakaryoblast cell line from a patient with Down syndrome-acute megakaryoblastic leukemia (DS-AMKL): in vitro differentiation of megakaryoblasts. |
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| Citation |
Blood 82 Suppl. 1:122a-122a(1993) |
|---|
| Abstract |
A cell line was established from a patient with DS-AMKL. The primary
leukemic cells were classified by electron microscopy as megakaryoblasts
by the presence of immunogold-labelled GpIIIa, demarcation membranes and
alpha granules. The cell line is growth factor dependent in liquid culture
in the presence of stem cell factor (SCF), and proliferates as a pure
population of undifferentiated blast cells. The cells have multiple
chromosomal abnormalities including monosomy 7, a finding initially
present in the megakaryoblasts at diagnosis in vivo. Interphase labelling
(FSIH) shows that all cells contained only one #7 chromosome. These cells
do not express the T-cell antigens CD3 and CD4, the B-cell antigens CD10
and CD19 or monocyte antigen CD14. However they are strongly positive for
the stem cell antigen CD34, the monomyeloid antigen CD33 and they also
express the megakaryocyte lineage antigens CD41, CD61 and CD7.
|
|---|
| Cell lines |
CVCL_S965; HSC-GRW |
|---|
