Cellosaurus cell line U2OS-LacO-I-SceI-TetO (CVCL

Cross-references
Cell line name	U2OS-LacO-I-SceI-TetO
Accession	CVCL_ZC51
Resource Identification Initiative	To cite this cell line use: U2OS-LacO-I-SceI-TetO (RRID:CVCL_ZC51)
Comments	Population: Caucasian. Characteristics: Cell line designed to probe the role of chromatin condensation in DNA damage response (DDR) using a lac-repressor/operator-tethering system (Kerafast=ENH105-FP). Characteristics: Contains 2 integrated copies of a construct that consist of a I-Scel endonuclease cut site (I-SceIcs) sandwiched between 256 copies of the lac operator (lacO) and 96 copies of the tet operator (tetO) (Kerafast=ENH105-FP). Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations	Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease	Osteosarcoma (NCIt: C9145) Osteosarcoma (ORDO: Orphanet_668)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0042 (U2OS)
Sex of cell	Female
Age at sampling	15Y
Category	Cancer cell line
Publications	PubMed=25464843; DOI=10.1016/j.celrep.2014.10.060; PMCID=PMC4267891 Burgess R.C., Burman B., Kruhlak M.J., Misteli T. Activation of DNA damage response signaling by condensed chromatin. Cell Rep. 9:1703-1717(2014)
Cell line collections (Providers)	Kerafast; ENH105-FP
Encyclopedic resources	Wikidata; Q98133788
Entry history
Entry creation	02-Jul-2020
Last entry update	05-Oct-2023
Version number	6