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Cellosaurus CTLL-2 NF-kappaB (CVCL_YU23)

[Text version]
Cell line name CTLL-2 NF-kappaB
Synonyms CTLL-2_NF-kappaB
Accession CVCL_YU23
Resource Identification Initiative To cite this cell line use: CTLL-2 NF-kappaB (RRID:CVCL_YU23)
Comments Characteristics: Reporter cell line for the measurement of the biological activity of a wide variety of cytokines.
Characteristics: Activated by NF-kappaB through a NF-kappaB response element fused to a secNanoLuc-T2A-mCherry construct.
Genetic integration: Method=Transfection/transduction; Gene=NanoLuc (Note=19 kDa ATP-independent bright luminescent protein derived from O.gracilirostris).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Cell type: T-cell; CL=CL_0000084.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6.
Hierarchy Parent: CVCL_0227 (CTLL-2)
Category Transformed cell line
Publications

PubMed=32094407; DOI=10.1038/s41598-020-60182-4; PMCID=PMC7040017
Mock J., Pellegrino C., Neri D.
A universal reporter cell line for bioactivity evaluation of engineered cytokine products.
Sci. Rep. 10:3234-3234(2020)

Cross-references
Encyclopedic resources Wikidata; Q93459998
Entry history
Entry creation12-Mar-2020
Last entry update10-Sep-2024
Version number7