Cellosaurus U2OS CHRM5 DAG-Nomad (CVCL_YK40)
| Cell line name | U2OS CHRM5 DAG-Nomad |
|---|---|
| Synonyms | DAG-NOMAD Muscarinic 5; DAGNomad M5; DAGNOMAD M5 |
| Accession | CVCL_YK40 |
| Resource Identification Initiative | To cite this cell line use: U2OS CHRM5 DAG-Nomad (RRID:CVCL_YK40) |
| Comments | Population: Caucasian. Characteristics: In absence of DAG, the fluorescent biosensor is localized in the cellular membrane. An increase in this second messenger concentration leads to change in the structural folding of Nomad biosensor that promotes its cellular relocation in the vesicular trafficking of the cells. Genetic integration: Method=Transfection/transduction; Gene=HGNC; 1954; CHRM5. Genetic integration: Method=Transfection/transduction; Gene=FPbase; JS838; TurboFP650 (Note=Far-red fluorescent protein derived from eqFP578). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979. |
| Sequence variations |
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| Disease | Osteosarcoma (NCIt: C9145) Osteosarcoma (ORDO: Orphanet_668) |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0042 (U2OS) |
| Sex of cell | Female |
| Age at sampling | 15Y |
| Category | Cancer cell line |
| Cross-references | |
| Cell line collections (Providers) | Innoprot; P70603 |
| Encyclopedic resources | Wikidata; Q98133740 |
| Entry history | |
| Entry creation | 19-Dec-2019 |
| Last entry update | 10-Sep-2024 |
| Version number | 10 |