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Cellosaurus UTA21.15 (CVCL_XF82)

[Text version]
Cell line name UTA21.15
Accession CVCL_XF82
Resource Identification Initiative To cite this cell line use: UTA21.15 (RRID:CVCL_XF82)
Comments Population: Caucasian.
Characteristics: Expresses very little CDKN1A when cultured in the presence of tetracycline, whereas CDKN1A expression is dramatically induced upon removal of tetracycline from the culture medium.
Characteristics: Transfected with an expression plasmid containing CKN1A (p21waf1) under control of a minimal CMV promoter fused to tetracycline operator sequences.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; 1784; CDKN1A.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_XF79 (UTA6)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Publications

PubMed=9484832; DOI=10.1038/sj.onc.1201558
Medema R.H., Klompmaker R., Smits V.A.J., Rijksen G.
p21waf1 can block cells at two points in the cell cycle, but does not interfere with processive DNA-replication or stress-activated kinases.
Oncogene 16:431-441(1998)

PubMed=10913154; DOI=10.1074/jbc.M005437200
Smits V.A.J., Klompmaker R., Vallenius T., Rijksen G., Makela T.P., Medema R.H.
p21 inhibits Thr161 phosphorylation of Cdc2 to enforce the G2 DNA damage checkpoint.
J. Biol. Chem. 275:30638-30643(2000)

Cross-references
Encyclopedic resources Wikidata; Q98134393
Entry history
Entry creation06-Sep-2019
Last entry update10-Sep-2024
Version number8