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Cellosaurus JCaM1/LckR154K (CVCL_XF43)

[Text version]
Cell line name JCaM1/LckR154K
Synonyms JRK 154.6
Accession CVCL_XF43
Resource Identification Initiative To cite this cell line use: JCaM1/LckR154K (RRID:CVCL_XF43)
Comments Population: Caucasian.
Characteristics: Parent cell line is deficient for LCK activity due to a deletion of exon 7. This cell line with a transfected LCK with the p.Arg154Lys substitution within the SH2 domain does not have a restored signaling activity (PubMed=8626636).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; 6524; LCK (Note=With p.Arg154Lys).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: T-cell; CL=CL_0000084.
Sequence variations
  • Mutation; HGNC; 959; BAX; Simple; p.Glu41Argfs*19 (c.121delG); ClinVar=VCV000009512; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 959; BAX; Simple; p.Glu41Glyfs*33 (c.121dupG); ClinVar=VCV000009511; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 16712; FBXW7; Simple; p.Arg505Cys (c.1513C>T); ClinVar=VCV000069961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6079; INPP5D; Simple; p.Gln345Ter (c.1033C>T); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6079; INPP5D; Simple; c.1097+1065_1097+1112del47; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 7325; MSH2; Simple; p.Arg711Ter (c.2131C>T); ClinVar=VCV000090903; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 7329; MSH6; Simple; p.Phe1088Serfs*2 (c.3261delC); ClinVar=VCV000089363; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11491; SYK; Simple; p.Met34Hisfs*3 (c.98_99insG); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg196Ter (c.586C>T); ClinVar=VCV000043589; Zygosity=Heterozygous (from parent cell line).
Disease Childhood T acute lymphoblastic leukemia (NCIt: C7953)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_XF41 (JCaM1/TAg)
Sex of cell Male
Age at sampling 14Y
Category Cancer cell line
Publications

PubMed=8626636; DOI=10.1074/jbc.271.17.9976
Straus D.B., Chan A.C.-Y., Patai B., Weiss A.
SH2 domain function is essential for the role of the Lck tyrosine kinase in T cell receptor signal transduction.
J. Biol. Chem. 271:9976-9981(1996)

PubMed=11231305; DOI=10.1046/j.1432-1327.2001.02028.x
Livolsi A., Busuttil V., Imbert V., Abraham R.T., Peyron J.-F.
Tyrosine phosphorylation-dependent activation of NF-kappa B Requirement for p56 LCK and ZAP-70 protein tyrosine kinases.
Eur. J. Biochem. 268:1508-1515(2001)

Cross-references
Cell line databases/resources cancercelllines; CVCL_XF43
Encyclopedic resources Wikidata; Q94326963
Entry history
Entry creation06-Sep-2019
Last entry update10-Sep-2024
Version number11