ID   JCaM1/LckR154K
AC   CVCL_XF43
SY   JRK 154.6
DR   cancercelllines; CVCL_XF43
DR   Wikidata; Q94326963
RX   PubMed=8626636;
RX   PubMed=11231305;
CC   Population: Caucasian.
CC   Characteristics: Parent cell line is deficient for LCK activity due to a deletion of exon 7. This cell line with a transfected LCK with the p.Arg154Lys substitution within the SH2 domain does not have a restored signaling activity (PubMed=8626636).
CC   Sequence variation: Mutation; HGNC; 959; BAX; Simple; p.Glu41Argfs*19 (c.121delG); ClinVar=VCV000009512; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 959; BAX; Simple; p.Glu41Glyfs*33 (c.121dupG); ClinVar=VCV000009511; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 16712; FBXW7; Simple; p.Arg505Cys (c.1513C>T); ClinVar=VCV000069961; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 6079; INPP5D; Simple; p.Gln345Ter (c.1033C>T); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 6079; INPP5D; Simple; c.1097+1065_1097+1112del47; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 7325; MSH2; Simple; p.Arg711Ter (c.2131C>T); ClinVar=VCV000090903; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 7329; MSH6; Simple; p.Phe1088Serfs*2 (c.3261delC); ClinVar=VCV000089363; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11491; SYK; Simple; p.Met34Hisfs*3 (c.98_99insG); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Arg196Ter (c.586C>T); ClinVar=VCV000043589; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; 6524; LCK (Note=With p.Arg154Lys).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
CC   Cell type: T-cell; CL=CL_0000084.
DI   NCIt; C7953; Childhood T acute lymphoblastic leukemia
DI   ORDO; Orphanet_99861; Precursor T-cell acute lymphoblastic leukemia
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_XF41 ! JCaM1/TAg
SX   Male
AG   14Y
CA   Cancer cell line
DT   Created: 06-09-19; Last updated: 10-09-24; Version: 11
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RX   PubMed=8626636; DOI=10.1074/jbc.271.17.9976;
RA   Straus D.B., Chan A.C.-Y., Patai B., Weiss A.;
RT   "SH2 domain function is essential for the role of the Lck tyrosine
RT   kinase in T cell receptor signal transduction.";
RL   J. Biol. Chem. 271:9976-9981(1996).
//
RX   PubMed=11231305; DOI=10.1046/j.1432-1327.2001.02028.x;
RA   Livolsi A., Busuttil V., Imbert V., Abraham R.T., Peyron J.-F.;
RT   "Tyrosine phosphorylation-dependent activation of NF-kappa B.
RT   Requirement for p56 LCK and ZAP-70 protein tyrosine kinases.";
RL   Eur. J. Biochem. 268:1508-1515(2001).
//