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Cellosaurus BU3 NKX2-1-GFP SFTPC-tdTomato (CVCL_WN82)

[Text version]
Cell line name BU3 NKX2-1-GFP SFTPC-tdTomato
Synonyms BU3 NKX2-1GFP;SFTPCtdTomato; BU3-NGST; BU3 NGST; BU3NGST
Accession CVCL_WN82
Resource Identification Initiative To cite this cell line use: BU3 NKX2-1-GFP SFTPC-tdTomato (RRID:CVCL_WN82)
Comments From: Boston University School of Medicine; Boston; USA.
Population: Caucasian.
Characteristics: Using CRISPR/Cas9 a 2A-eGFP-pA was inserted after the NKX2-1 coding sequence and using TALENs a tdTomato-pA-loxP-PGKp-PuroR-pA-loxP was inserted at the SFTPC translational start site.
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=TALEN; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Genetic integration: Method=TALEN; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_JW26 (CREM003i-BU3C2)
Sex of cell Male
Age at sampling 32Y
Category Induced pluripotent stem cell
Web pages https://stemcellbank.bu.edu/Catalog/Item/Details/509
Publications

PubMed=28965766; DOI=10.1016/j.stem.2017.08.014; PMCID=PMC5755620
Jacob A., Morley M., Hawkins F., McCauley K.B., Jean J.-C., Heins H., Na C.-L., Weaver T.E., Vedaie M., Hurley K., Hinds A., Russo S.J., Kook S., Zacharias W., Ochs M., Traber K., Quinton L.J., Crane A.M., Davis B.R., White F.V., Wambach J., Whitsett J.A., Cole F.S., Morrisey E.E., Guttentag S.H., Beers M.F., Kotton D.N.
Differentiation of human pluripotent stem cells into functional lung alveolar epithelial cells.
Cell Stem Cell 21:472-488.e10(2017)

PubMed=31924806; DOI=10.1038/s41467-019-13916-6; PMCID=PMC6954238
Mithal A., Capilla A., Heinze D., Berical A., Villacorta-Martin C., Vedaie M., Jacob A., Abo K., Szymaniak A.D., Peasley M., Stuffer A., Mahoney J., Kotton D.N., Hawkins F., Mostoslavsky G.
Generation of mesenchyme free intestinal organoids from human induced pluripotent stem cells.
Nat. Commun. 11:215.1-215.15(2020)

Cross-references
Encyclopedic resources Wikidata; Q93433163
Entry history
Entry creation24-May-2019
Last entry update10-Sep-2024
Version number7