Cellosaurus BU3 NKX2-1-GFP SFTPC-tdTomato (CVCL_WN82)
Cell line name | BU3 NKX2-1-GFP SFTPC-tdTomato |
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Synonyms | BU3 NKX2-1GFP;SFTPCtdTomato; BU3-NGST; BU3 NGST; BU3NGST |
Accession | CVCL_WN82 |
Resource Identification Initiative | To cite this cell line use: BU3 NKX2-1-GFP SFTPC-tdTomato (RRID:CVCL_WN82) |
Comments | From: Boston University School of Medicine; Boston; USA. Population: Caucasian. Characteristics: Using CRISPR/Cas9 a 2A-eGFP-pA was inserted after the NKX2-1 coding sequence and using TALENs a tdTomato-pA-loxP-PGK-Puro-pA-loxP was inserted at the SFTPC translational start site. Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: tdTomato, a bright red fluorescent protein derived from drFP583 (DsRed). Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178. |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
Hierarchy | Parent: CVCL_JW26 (CREM003i-BU3C2) |
Sex of cell | Male |
Age at sampling | 32Y |
Category | Induced pluripotent stem cell |
Web pages | https://stemcellbank.bu.edu/Catalog/Item/Details/509 |
Publications | PubMed=28965766; DOI=10.1016/j.stem.2017.08.014 PubMed=31924806; DOI=10.1038/s41467-019-13916-6 |
Cross-references | |
Encyclopedic resources | Wikidata; Q93433163 |
Entry history | |
Entry creation | 24-May-2019 |
Last entry update | 30-Jan-2024 |
Version number | 6 |