ID   BU3 NKX2-1-GFP SFTPC-tdTomato
AC   CVCL_WN82
SY   BU3 NKX2-1GFP;SFTPCtdTomato; BU3-NGST; BU3 NGST; BU3NGST
DR   Wikidata; Q93433163
RX   PubMed=28965766;
RX   PubMed=31924806;
WW   https://stemcellbank.bu.edu/Catalog/Item/Details/509
CC   From: Boston University School of Medicine; Boston; USA.
CC   Population: Caucasian.
CC   Characteristics: Using CRISPR/Cas9 a 2A-eGFP-pA was inserted after the NKX2-1 coding sequence and using TALENs a tdTomato-pA-loxP-PGK-Puro-pA-loxP was inserted at the SFTPC translational start site.
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transfected with: tdTomato, a bright red fluorescent protein derived from drFP583 (DsRed).
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_JW26 ! CREM003i-BU3C2
SX   Male
AG   32Y
CA   Induced pluripotent stem cell
DT   Created: 24-05-19; Last updated: 30-01-24; Version: 6
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RX   PubMed=28965766; DOI=10.1016/j.stem.2017.08.014;
RA   Jacob A., Morley M., Hawkins F., McCauley K.B., Jean J.-C., Heins H.,
RA   Na C.-L., Weaver T.E., Vedaie M., Hurley K., Hinds A., Russo S.J.,
RA   Kook S., Zacharias W., Ochs M., Traber K., Quinton L.J., Crane A.M.,
RA   Davis B.R., White F.V., Wambach J., Whitsett J.A., Cole F.S.,
RA   Morrisey E.E., Guttentag S.H., Beers M.F., Kotton D.N.;
RT   "Differentiation of human pluripotent stem cells into functional lung
RT   alveolar epithelial cells.";
RL   Cell Stem Cell 21:472-488.e10(2017).
//
RX   PubMed=31924806; DOI=10.1038/s41467-019-13916-6;
RA   Mithal A., Capilla A., Heinze D., Berical A., Villacorta-Martin C.,
RA   Vedaie M., Jacob A., Abo K., Szymaniak A.D., Peasley M., Stuffer A.,
RA   Mahoney J., Kotton D.N., Hawkins F., Mostoslavsky G.;
RT   "Generation of mesenchyme free intestinal organoids from human induced
RT   pluripotent stem cells.";
RL   Nat. Commun. 11:215.1-215.15(2020).
//