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Cellosaurus N2a-PK1 (CVCL_WI10)

[Text version]
Cell line name N2a-PK1
Synonyms N2aPK1; PK1
Accession CVCL_WI10
Resource Identification Initiative To cite this cell line use: N2a-PK1 (RRID:CVCL_WI10)
Comments Biotechnology: Used in the scrapie cell (SC) assay. This susceptible cell line is exposed to mouse prion-containing samples for 3 days, grown to confluence and split 1:10 three times. The proportion of PrPSc-containing cells is determined with automated counting equipment.
Characteristics: Highly susceptible to infection by prion (PrPsc).
Derived from site: In situ; Peripheral nervous system; UBERON=UBERON_0000010.
Cell type: Neural crest derived neuroblast; CL=CL_0002676.
Disease Mouse neuroblastoma (NCIt: C21993)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: A/J.
Hierarchy Parent: CVCL_WI09 (N2a-PD88)
Sex of cell Male
Category Cancer cell line
Publications

PubMed=14504404; DOI=10.1073/pnas.1834432100; PMCID=PMC208815
Klohn P.-C., Stoltze L., Flechsig E., Enari M., Weissmann C.
A quantitative, highly sensitive cell-based infectivity assay for mouse scrapie prions.
Proc. Natl. Acad. Sci. U.S.A. 100:11666-11671(2003)

PubMed=18077360; DOI=10.1073/pnas.0710054104; PMCID=PMC2409240
Mahal S.P., Baker C.A., Demczyk C.A., Smith E.W., Julius C., Weissmann C.
Prion strain discrimination in cell culture: the cell panel assay.
Proc. Natl. Acad. Sci. U.S.A. 104:20908-20913(2007)

Cross-references
Encyclopedic resources Wikidata; Q95991905
Entry history
Entry creation24-May-2019
Last entry update29-Jun-2023
Version number5