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Cellosaurus CHO-gmt4 (CVCL_W354)

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Cell line name CHO-gmt4
Synonyms JW152
Accession CVCL_W354
Resource Identification Initiative To cite this cell line use: CHO-gmt4 (RRID:CVCL_W354)
Comments Group: Patented cell line.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); PTA-9657.
Characteristics: Lacks N-acetylglucosaminyltransferase I activity.
Selected for resistance to: UniProtKB; P02879; Ricinus communis ricin.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Mutation; UniProtKB; Q924C0; Mgat1; Simple; p.Gln339Ter (c.1015C>T); Zygosity=Unspecified (PubMed=20346410).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_0214 (CHO-K1)
Children:
CVCL_A8SP (CHO-gmt4D)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=20346410; DOI=10.1016/j.ymben.2010.03.002
John Soo Yan Goh, Pei-Qing Zhang, Kah Fai Chan, May May Lee, Sing Fee Lim, Zhi-Wei Song;
RCA-I-resistant CHO mutant cells have dysfunctional GnT I and expression of normal GnT I in these mutants enhances sialylation of recombinant erythropoietin.
Metab. Eng. 12:360-368(2010)

CLPUB00638
John Soo Yan Goh;
Applications of a novel CHO glycosylation mutant.
Thesis PhD (2014); National University of Singapore; Singapore; Singapore

PubMed=24166780; DOI=10.1002/biot.201300301
John Soo Yan Goh, Ying-Wei Liu, Hai-Feng Liu, Kah Fai Chan, Corrine Wan, Gavin Teo, Xiang-Shan Zhou, Fu-Sheng Xie, Pei-Qing Zhang, Yuan-Xing Zhang, Zhi-Wei Song;
Highly sialylated recombinant human erythropoietin production in large-scale perfusion bioreactor utilizing CHO-gmt4 (JW152) with restored GnT I function.
Biotechnol. J. 9:100-109(2014)

Patent=US9243235
Zhi-Wei Song;
Method of producing recombinant proteins with mannose-terminated N-glycans.
Patent number US9243235, 26-Jan-2016

Cross-references
Encyclopedic resources Wikidata; Q54899194
Entry history
Entry creation16-Apr-2014
Last entry update30-Jan-2024
Version number11