ID   CHO-gmt4
AC   CVCL_W354
SY   JW152
DR   Wikidata; Q54899194
RX   CelloPub=CLPUB00638;
RX   Patent=US9243235;
RX   PubMed=20346410;
RX   PubMed=24166780;
CC   Group: Patented cell line.
CC   Registration: International Depositary Authority, American Type Culture Collection (ATCC); PTA-9657.
CC   Characteristics: Lacks N-acetylglucosaminyltransferase I activity.
CC   Selected for resistance to: UniProtKB; P02879; Ricinus communis ricin.
CC   Sequence variation: Mutation; UniProtKB; Q924C0; Mgat1; Simple; p.Gln339Ter (c.1015C>T); Zygosity=Unspecified (PubMed=20346410).
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_0214 ! CHO-K1
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 16-04-14; Last updated: 30-01-24; Version: 11
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RX   CelloPub=CLPUB00638;
RA   Goh J.S.Y.;
RT   "Applications of a novel CHO glycosylation mutant.";
RL   Thesis PhD (2014), National University of Singapore, Singapore.
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RX   Patent=US9243235;
RA   Song Z.-W.;
RT   "Method of producing recombinant proteins with mannose-terminated
RT   N-glycans.";
RL   Patent number US9243235, 26-Jan-2016.
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RX   PubMed=20346410; DOI=10.1016/j.ymben.2010.03.002;
RA   Goh J.S.Y., Zhang P.-Q., Chan K.F., Lee M.M., Lim S.F., Song Z.-W.;
RT   "RCA-I-resistant CHO mutant cells have dysfunctional GnT I and
RT   expression of normal GnT I in these mutants enhances sialylation of
RT   recombinant erythropoietin.";
RL   Metab. Eng. 12:360-368(2010).
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RX   PubMed=24166780; DOI=10.1002/biot.201300301;
RA   Goh J.S.Y., Liu Y.-W., Liu H.-F., Chan K.F., Wan C., Teo G., Zhou X.-S.,
RA   Xie F.-S., Zhang P.-Q., Zhang Y.-X., Song Z.-W.;
RT   "Highly sialylated recombinant human erythropoietin production in
RT   large-scale perfusion bioreactor utilizing CHO-gmt4 (JW152) with
RT   restored GnT I function.";
RL   Biotechnol. J. 9:100-109(2014).
//